PMID- 17386724 OWN - NLM STAT- MEDLINE DCOM- 20070607 LR - 20091119 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 586 IP - 1-2 DP - 2007 Mar 14 TI - Validation and comparison of the Copan Milk Test and Delvotest SP-NT for the detection of antimicrobials in milk. PG - 280-3 AB - The Delvotest SP-NT and Copan Milk Test, two microbiological tests designed for screening antimicrobial substances in milk were compared and validated. The performance criteria described by the European Decision 2002/657/EC were used for the study. Both tests were evaluated with visual and automated reading (scanner) and the validation was performed on 10 different antibiotics (penicillin-G, cloxacillin, sulfamethazine, sulfadiazine, oxytetracycline, gentamicin, cephalexin, cefquinome, dihydrostreptomycin and trimethoprim). Both tests were found to detect penicillin, cloxacillin, sulfamethazine, sulfadiazine, cephalexin and gentamicin at or below the EU maximum residue limits (MRLs). Some other antibiotics such as oxytetracycline, dihydrostreptomycin, trimethoprim and cefquinome were not detected or only with a low sensitivity. Both tests were found easy to use, robust and fulfilled EU requirements. FAU - Le Breton, Marie-Helene AU - Le Breton MH AD - Nestle Research Center, Nestle Ltd., P.O. Box 44, CH-1000 Lausanne 26, Switzerland. marie-helene.le-breton@rdls.nestle.com FAU - Savoy-Perroud, Marie-Claude AU - Savoy-Perroud MC FAU - Diserens, Jean-Marc AU - Diserens JM LA - eng PT - Comparative Study PT - Journal Article DEP - 20061202 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Anti-Bacterial Agents) RN - 0 (Anti-Infective Agents) SB - IM MH - Animals MH - Anti-Bacterial Agents/*analysis/chemistry MH - Anti-Infective Agents/*analysis/chemistry MH - Cattle MH - Chemistry Techniques, Analytical/instrumentation/methods MH - Drug Residues/analysis MH - Food Analysis/instrumentation/*methods MH - Milk/*metabolism MH - Reproducibility of Results MH - Research Design MH - Sensitivity and Specificity EDAT- 2007/03/28 09:00 MHDA- 2007/06/08 09:00 CRDT- 2007/03/28 09:00 PHST- 2006/06/01 00:00 [received] PHST- 2006/11/23 00:00 [revised] PHST- 2006/11/25 00:00 [accepted] PHST- 2007/03/28 09:00 [pubmed] PHST- 2007/06/08 09:00 [medline] PHST- 2007/03/28 09:00 [entrez] AID - S0003-2670(06)02273-2 [pii] AID - 10.1016/j.aca.2006.11.060 [doi] PST - ppublish SO - Anal Chim Acta. 2007 Mar 14;586(1-2):280-3. doi: 10.1016/j.aca.2006.11.060. Epub 2006 Dec 2.