PMID- 17386921
OWN - NLM
STAT- MEDLINE
DCOM- 20070628
LR  - 20070411
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 356
IP  - 4
DP  - 2007 May 18
TI  - Leptin-induced matrix metalloproteinase-2 secretion is suppressed by 
      trans-10,cis-12 conjugated linoleic acid.
PG  - 955-60
AB  - It has long been recognized that leptin, a hormone made by adipocytes, is an 
      important circulating signal for the regulation of body weight. In addition, 
      matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein 
      which promotes multi-cellular adipose clusters, is up-regulated in obesity. The 
      present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic 
      acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The 
      result showed that expressions of adipocyte marker proteins were significantly 
      reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. 
      Interestingly, MMP-2 secretion was significantly increased by leptin-treated 
      cultures, thereby leading to accelerate adipocyte differentiation, indicating 
      that MMP-2 was a necessary mediator of adipogenesis. However, increasing 
      concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and 
      triglyceride (TG) content. These findings provide support for a role for t-CLA in 
      the regulation of metabolism in leptin-induced adipose tissue development.
FAU - Moon, Hyun-Seuk
AU  - Moon HS
AD  - School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, 
      Republic of Korea.
FAU - Lee, Hong-Gu
AU  - Lee HG
FAU - Seo, Ji-Hye
AU  - Seo JH
FAU - Chung, Chung-Soo
AU  - Chung CS
FAU - Guo, Ding-Ding
AU  - Guo DD
FAU - Kim, Tae-Gyu
AU  - Kim TG
FAU - Choi, Yun-Jaie
AU  - Choi YJ
FAU - Cho, Chong-Su
AU  - Cho CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070321
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Drug Combinations)
RN  - 0 (Leptin)
RN  - 0 (Linoleic Acids, Conjugated)
RN  - 0 (trans-10,cis-12-conjugated linoleic acid)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/drug effects/*enzymology
MH  - Adipogenesis/drug effects/*physiology
MH  - Animals
MH  - Drug Combinations
MH  - Leptin/*administration & dosage
MH  - Linoleic Acids, Conjugated/*administration & dosage
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Mice
MH  - Signal Transduction/drug effects/*physiology
EDAT- 2007/03/28 09:00
MHDA- 2007/06/29 09:00
CRDT- 2007/03/28 09:00
PHST- 2007/03/12 00:00 [received]
PHST- 2007/03/14 00:00 [accepted]
PHST- 2007/03/28 09:00 [pubmed]
PHST- 2007/06/29 09:00 [medline]
PHST- 2007/03/28 09:00 [entrez]
AID - S0006-291X(07)00563-3 [pii]
AID - 10.1016/j.bbrc.2007.03.068 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 May 18;356(4):955-60. doi: 
      10.1016/j.bbrc.2007.03.068. Epub 2007 Mar 21.