PMID- 17388926
OWN - NLM
STAT- MEDLINE
DCOM- 20070905
LR  - 20131121
IS  - 0007-0963 (Print)
IS  - 0007-0963 (Linking)
VI  - 156
IP  - 5
DP  - 2007 May
TI  - Protection from photodamage by topical application of caffeine after ultraviolet 
      irradiation.
PG  - 957-64
AB  - BACKGROUND: Characterization of mechanisms that can reverse residual damage from 
      prior skin exposure to ultraviolet (UV) would be of considerable biological and 
      therapeutic interest. Topical caffeine application to mouse skin that had 
      previously been treated with UV has been shown to inhibit the subsequent 
      development of squamous cell carcinomas. OBJECTIVES: We used an established mouse 
      photodamage model to investigate other possible effects of topical caffeine 
      application after UV. METHODS: SKH-1 hairless mice were treated with ultraviolet 
      B (UVB) followed immediately by topical application of caffeine or vehicle three 
      times weekly for 11 weeks. RESULTS: Caffeine applied topically after UV treatment 
      resulted in a significant decrease in UV-induced skin roughness/transverse 
      rhytides as assessed by treatment-blinded examiners. Histologically, topical 
      caffeine application after a single dose of UVB more than doubled the number of 
      apoptotic keratinocytes as evaluated by sunburn cell formation, caspase 3 
      cleavage and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end 
      labelling (TUNEL) staining. A trend towards decreased solar elastosis was noted 
      in the caffeine-treated group although this was not statistically significant. 
      Other histological parameters including epidermal hyperplasia, solar elastosis 
      and angiogenesis were increased in mice treated with UV but topical application 
      of caffeine did not alter these particular UV effects. CONCLUSIONS: These 
      findings support the concept that topical application of caffeine to mouse skin 
      after UV irradiation promotes the deletion of DNA-damaged keratinocytes and may 
      partially diminish photodamage as well as photocarcinogenesis.
FAU - Koo, S-W
AU  - Koo SW
AD  - Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard 
      Medical School, Boston, MA 02129, USA.
FAU - Hirakawa, S
AU  - Hirakawa S
FAU - Fujii, S
AU  - Fujii S
FAU - Kawasumi, M
AU  - Kawasumi M
FAU - Nghiem, P
AU  - Nghiem P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070328
PL  - England
TA  - Br J Dermatol
JT  - The British journal of dermatology
JID - 0004041
RN  - 0 (Dermatologic Agents)
RN  - 3G6A5W338E (Caffeine)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Apoptosis/*drug effects/radiation effects
MH  - Caffeine/*pharmacology
MH  - Dermatologic Agents/*pharmacology
MH  - Keratinocytes/*drug effects/radiation effects
MH  - Mice
MH  - Mice, Hairless
MH  - Models, Animal
MH  - Radiation Injuries, Experimental/*drug therapy
MH  - Skin/*drug effects/radiation effects
MH  - Ultraviolet Rays
EDAT- 2007/03/29 09:00
MHDA- 2007/09/06 09:00
CRDT- 2007/03/29 09:00
PHST- 2007/03/29 09:00 [pubmed]
PHST- 2007/09/06 09:00 [medline]
PHST- 2007/03/29 09:00 [entrez]
AID - BJD7812 [pii]
AID - 10.1111/j.1365-2133.2007.07812.x [doi]
PST - ppublish
SO  - Br J Dermatol. 2007 May;156(5):957-64. doi: 10.1111/j.1365-2133.2007.07812.x. 
      Epub 2007 Mar 28.