PMID- 17389653
OWN - NLM
STAT- MEDLINE
DCOM- 20070912
LR  - 20161124
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 17
IP  - 7
DP  - 2007 Jul
TI  - Trypanosome trans-sialidase mediates neuroprotection against oxidative stress, 
      serum/glucose deprivation, and hypoxia-induced neurite retraction in 
      Trk-expressing PC12 cells.
PG  - 725-34
AB  - Trypanosome trans-sialidase (TS) is a sialic acid-transferring enzyme and a novel 
      ligand of tyrosine kinase (TrkA) receptors but not of neurotrophin receptor 
      p75NTR. Here, we show that TS targets TrkB receptors on TrkB-expressing 
      pheochromocytoma PC12 cells and colocalizes with TrkB receptor internalization 
      and phosphorylation (pTrkB). Wild-type TS but not the catalytically inactive 
      mutant TSDeltaAsp98-Glu induces pTrkB and mediates cell survival responses 
      against death caused by oxidative stress in TrkA- and TrkB-expressing cells like 
      those seen with nerve growth factor (NGF) and brain-derived neurotrophic factor 
      (BDNF). These same effects are not observed in Trk deficient PC12(nnr5) cells, 
      but are re-established in PC12(nnr5) cells stably transfected with TrkA or TrkB, 
      are partially blocked by inhibitors of tyrosine kinase (K-252a), 
      mitogen-activated protein/mitogen-activated kinase (PD98059) and completely 
      blocked by LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI3K). Both 
      TrkA- and TrkB-expressing cells pretreated with TS or their natural ligands are 
      protected against cell death caused by serum/glucose deprivation or from 
      hypoxia-induced neurite retraction. The cell survival effects of NGF and BDNF 
      against oxidative stress are significantly inhibited by the neuraminidase 
      inhibitor, Tamiflu. Together, these observations suggest that trypanosome TS 
      mimics neurotrophic factors in cell survival responses against oxidative stress, 
      hypoxia-induced neurite retraction and serum/glucose deprivation.
FAU - Woronowicz, Alicja
AU  - Woronowicz A
AD  - Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, 
      Canada K7L3N6.
FAU - Amith, Schammim Ray
AU  - Amith SR
FAU - Davis, Vanessa W
AU  - Davis VW
FAU - Jayanth, Preethi
AU  - Jayanth P
FAU - De Vusser, Kristof
AU  - De Vusser K
FAU - Laroy, Wouter
AU  - Laroy W
FAU - Contreras, Roland
AU  - Contreras R
FAU - Meakin, Susan O
AU  - Meakin SO
FAU - Szewczuk, Myron R
AU  - Szewczuk MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070327
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glycoproteins)
RN  - 20O93L6F9H (Oseltamivir)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.2.1.- (trans-sialidase)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Enzyme Inhibitors/pharmacology
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Glucose/*metabolism
MH  - Glycoproteins/*metabolism
MH  - Hypoxia
MH  - Nerve Growth Factor/metabolism
MH  - Neuraminidase/*metabolism
MH  - Oseltamivir/pharmacology
MH  - *Oxidative Stress
MH  - PC12 Cells
MH  - Rats
MH  - Receptor, trkB/*metabolism
MH  - Serum/*metabolism
MH  - Trypanosoma cruzi/*metabolism
EDAT- 2007/03/29 09:00
MHDA- 2007/09/13 09:00
CRDT- 2007/03/29 09:00
PHST- 2007/03/29 09:00 [pubmed]
PHST- 2007/09/13 09:00 [medline]
PHST- 2007/03/29 09:00 [entrez]
AID - cwm034 [pii]
AID - 10.1093/glycob/cwm034 [doi]
PST - ppublish
SO  - Glycobiology. 2007 Jul;17(7):725-34. doi: 10.1093/glycob/cwm034. Epub 2007 Mar 
      27.