PMID- 17390664
OWN - NLM
STAT- MEDLINE
DCOM- 20100513
LR  - 20131121
IS  - 1000-0593 (Print)
IS  - 1000-0593 (Linking)
VI  - 27
IP  - 1
DP  - 2007 Jan
TI  - [Spectrophotometric study on the interaction of acridine orange and heparin].
PG  - 116-9
AB  - The interaction of acridine orange (AO) and heparin was studied by 
      spectrophotometry. In the pH 2. 0 Britton-Robinson (B-R) buffer solution, AO had 
      a maximum absorption at 478 nm and 492 nm. After the addition of heparin, the 
      absorbance value at 492 nm decreased greatly and a new absorption peak at 453 nm 
      appeared, indicating a strong interaction taking place in the reaction solution. 
      Under the optimal condition, the decrease in absorbance at 492 nm was linear with 
      the concentration of heparin in the range of 3. 0-15.0 mg m L(-1). The m olar 
      absorptivity w as calculated to be E=l. 599X 10(5) L mol(-1) x cm(-1) and the 
      detection limit was 0. 236 mg x L(-1) (3sigma). The method was furthur applied to 
      the detection of heparin sodium injection samples with satisfactory results. The 
      binding number was calculated to be 1 : 3 with molar ratio method.
FAU - Sun, Wei
AU  - Sun W
AD  - College of Chemistry and Molecular Engineering, Qingdao University of Science and 
      Technology, Qingdao 266042, China. sunwei@qust.edu.cn
FAU - Ding, Ya-qin
AU  - Ding YQ
FAU - Teng, Wen-hui
AU  - Teng WH
FAU - Jiao, Kui
AU  - Jiao K
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Guang Pu Xue Yu Guang Pu Fen Xi
JT  - Guang pu xue yu guang pu fen xi = Guang pu
JID - 9424805
RN  - 9005-49-6 (Heparin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*analysis/chemistry
MH  - Heparin/*analysis/chemistry
MH  - Spectrophotometry/*methods
EDAT- 2007/03/30 09:00
MHDA- 2010/05/14 06:00
CRDT- 2007/03/30 09:00
PHST- 2007/03/30 09:00 [pubmed]
PHST- 2010/05/14 06:00 [medline]
PHST- 2007/03/30 09:00 [entrez]
PST - ppublish
SO  - Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Jan;27(1):116-9.