PMID- 17391368
OWN - NLM
STAT- MEDLINE
DCOM- 20070803
LR  - 20131121
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 271
IP  - 1
DP  - 2007 Jun
TI  - Growth and physiological response of Arthrobacter protophormiae RKJ100 toward 
      higher concentrations of o-nitrobenzoate and p-hydroxybenzoate.
PG  - 65-70
AB  - Bioremediation of sites that are heavily contaminated with pollutant chemicals is 
      a challenge as most of the microorganisms cannot tolerate higher concentrations 
      of toxic compounds. Only a few strains of the genus Pseudomonas have been studied 
      for their tolerance toward the higher concentrations of aromatic pollutant 
      compounds, a phenomenon that is accompanied by various physiological changes. In 
      the present study we have characterized the growth response and physiological 
      changes (adaptations) of a Gram-positive bacterium, Arthrobacter protophormiae 
      RKJ100, toward the higher concentrations of two aromatic compounds, viz. 
      o-nitrobenzoate (ONB) and p-hydroxybenzoate (PHB). Arthrobacter protophormiae 
      RKJ100 could utilize 30 mM ONB and 50 mM PHB as sole sources of carbon and 
      energy. It was capable of growth on higher concentrations of ONB (up to 200 mM) 
      and PHB (up to 150 mM) when the cells were pre-exposed to lower concentrations of 
      these compounds. The adaptive responses shown by the organism during growth on 
      higher concentrations of these compounds were evident from significant changes in 
      cellular fatty acid profiles. In addition, Bacterial Adhesion To Hydrocarbon 
      (BATH) assay and scanning electron microscopy showed substantial increase in cell 
      surface hydrophobicity and decrease in cell size of A. protophormiae RKJ100 when 
      grown on ONB and PHB as compared to succinate-grown cells.
FAU - Sharma, Narinder K
AU  - Sharma NK
AD  - Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
FAU - Pandey, Janmejay
AU  - Pandey J
FAU - Gupta, Nisha
AU  - Gupta N
FAU - Jain, Rakesh K
AU  - Jain RK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070328
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (2-nitrobenzoate)
RN  - 0 (Fatty Acids)
RN  - 0 (Nitrobenzoates)
RN  - 0 (Parabens)
RN  - 7440-44-0 (Carbon)
RN  - AB6MNQ6J6L (Succinic Acid)
RN  - JG8Z55Y12H (4-hydroxybenzoic acid)
SB  - IM
MH  - *Adaptation, Physiological
MH  - Arthrobacter/cytology/*drug effects/*growth & development/metabolism
MH  - Bacterial Adhesion/drug effects
MH  - Carbon/metabolism
MH  - Drug Tolerance
MH  - Energy Metabolism
MH  - Fatty Acids/metabolism
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Microscopy, Electron, Scanning
MH  - Nitrobenzoates/metabolism/*pharmacology
MH  - Parabens/metabolism/*pharmacology
MH  - Succinic Acid/metabolism
EDAT- 2007/03/30 09:00
MHDA- 2007/08/04 09:00
CRDT- 2007/03/30 09:00
PHST- 2007/03/30 09:00 [pubmed]
PHST- 2007/08/04 09:00 [medline]
PHST- 2007/03/30 09:00 [entrez]
AID - FML697 [pii]
AID - 10.1111/j.1574-6968.2007.00697.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2007 Jun;271(1):65-70. doi: 
      10.1111/j.1574-6968.2007.00697.x. Epub 2007 Mar 28.