PMID- 1739137
OWN - NLM
STAT- MEDLINE
DCOM- 19920318
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 140
IP  - 2
DP  - 1992 Feb
TI  - Establishment and characterization of five new human renal tumor xenografts.
PG  - 483-95
AB  - Ten different human renal cell carcinoma (RCC) primary tumors were xenografted 
      into BALB/c nu/nu mice. Five of the tumors (NU-10, NU-12, NU-20, NU-22, and 
      NU-28) gave rise to serially transplantable tumors that were further 
      characterized. Histology, DNA index, immunohistochemical characteristics, growth 
      rate, and clonogenic potential were followed from primary tumor to the 5th to 
      15th transplant passage. Only one of the tumors (NU-20) showed remarkable 
      instability for all tested parameters in the first five transplant passages. 
      Histology of the other tumors was essentially the same to the histology of the 
      primary tumors, although differences between human and host-derived vessels were 
      apparent. DNA index values in general showed a trend toward an aneuploid 
      character of the xenografts. Immunohistochemical analyses showed a loss of 
      intensity of staining but a concomitant rise in the fraction of positively 
      staining cells with antibodies against cytokeratins, vimentin, tumor-associated 
      antigens, and human leukocyte antigen (HLA) class I antigens. Human leukocyte 
      antigen class II antigen expression showed a loss of intensity as well as a 
      decrease in the fraction of positive cells. Tumor doubling time was lowest in 
      transplant passage number 0, and stable growth was noticed in transplant passages 
      1 through 4. Clonogenic potential of four of the lines was higher for the 
      xenografts than for the primary tumors. The authors conclude that, on 
      xenografting, histologic characteristics of the primary tumor are essentially 
      conserved. Progression in the first transplant passages, however, results in 
      tumors with a more aggressive character.
FAU - Beniers, A J
AU  - Beniers AJ
AD  - Department of Urology, University Hospital Nijmegen, The Netherlands.
FAU - Peelen, W P
AU  - Peelen WP
FAU - Schaafsma, H E
AU  - Schaafsma HE
FAU - Beck, J L
AU  - Beck JL
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - Debruyne, F M
AU  - Debruyne FM
FAU - Schalken, J A
AU  - Schalken JA
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Carcinoma, Renal Cell/genetics/*pathology/secondary
MH  - Cell Division
MH  - DNA, Neoplasm/analysis
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Kidney Neoplasms/genetics/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Middle Aged
MH  - Neoplasm Transplantation/*pathology
MH  - Tumor Cells, Cultured
MH  - Tumor Stem Cell Assay
PMC - PMC1886418
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
PMCR- 1992/08/01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
PHST- 1992/08/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1992 Feb;140(2):483-95.