PMID- 17392495
OWN - NLM
STAT- MEDLINE
DCOM- 20070509
LR  - 20171116
IS  - 1535-3702 (Print)
IS  - 1535-3699 (Linking)
VI  - 232
IP  - 4
DP  - 2007 Apr
TI  - Platelet expression of CD40/CD40 ligand and its relation to inflammatory markers 
      and adhesion molecules in patients with atrial fibrillation.
PG  - 581-9
AB  - Recent studies suggest the importance of prothrombotic and proinflammatory 
      cascades in vascular thrombus formation. However, the impact of platelet CD40 and 
      CD40 ligand (CD40L) expression and its relation to inflammatory markers in atrial 
      clot formation have not yet been determined. Therefore, we studied a total of 40 
      patients. A total of 20 patients with persistent atrial fibrillation (AF) and 20 
      matched patients with sinus rhythm (SR) were included to quantify platelet 
      surface expression of CD40/CD40L, serum levels of intercellular adhesion 
      molecule-1 (ICAM), vascular adhesion molecule-1 (VCAM), high-sensitivity 
      C-reactive protein (hsCRP), and monocyte chemoattractant protein-1 (MCP-1). Using 
      fluorescence-activated cell sorting analysis, baseline CD40 expression (antibody 
      binding capacity [ABC]) was increased during AF (AF: 7776 +/- 8.46 ABC vs. SR: 
      7753 +/- 7.32 ABC; P < 0.05), whereas CD40L expression was not different. In 
      contrast to the effect of adenosine diphosphate, ex vivo stimulation with 
      thrombin receptor activating peptide (TRAP) increased CD40 and CD40L expression 
      in both groups. MCP-1, hsCRP, ICAM, and VCAM levels were significantly increased 
      during AF, reaching highest levels in patients with atrial thrombi. Importantly, 
      VCAM and MCP-1 were independent predictors for atrial thrombi (P < 0.05) using 
      multivariate analysis. In contrast to declining levels of hsCRP, levels of ICAM, 
      VCAM, MCP-1, and platelet CD40 expression remained elevated 5 weeks after 
      successful electrical direct current cardioversion (CV). In conclusion, 
      prothrombogenic markers are substantially elevated in patients with AF, reaching 
      highest levels in patients with AF and atrial thrombi. Interestingly, amounts of 
      adhesion molecules and platelet CD40 levels remain elevated even 5 weeks after 
      successful CV, which may imply a persistently increased risk for atrial thrombus 
      formation. In addition to hsCRP, MCP-1 and VCAM may serve as new biomarkers, 
      which may help to identify patients with an increased risk for thromboembolic 
      events.
FAU - Hammwohner, Matthias
AU  - Hammwohner M
AD  - Division of Cardiology, Department of Internal Medicine, University Hospital 
      Magdeburg, Leipzigerstr. 44, 39120 Magdeburg, Germany.
FAU - Ittenson, Annelore
AU  - Ittenson A
FAU - Dierkes, Jutta
AU  - Dierkes J
FAU - Bukowska, Alicja
AU  - Bukowska A
FAU - Klein, Helmut U
AU  - Klein HU
FAU - Lendeckel, Uwe
AU  - Lendeckel U
FAU - Goette, Andreas
AU  - Goette A
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Biomarkers)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Inflammation Mediators)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
SB  - IM
MH  - Adenosine Diphosphate/pharmacology
MH  - Atrial Fibrillation/complications/*metabolism
MH  - Biomarkers/metabolism
MH  - Blood Platelets/*metabolism
MH  - CD40 Antigens/*biosynthesis
MH  - CD40 Ligand/*biosynthesis
MH  - Cell Adhesion Molecules/*biosynthesis
MH  - Female
MH  - Follow-Up Studies
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Inflammation Mediators/*blood
MH  - Male
MH  - Middle Aged
MH  - Risk Factors
MH  - Thrombosis/etiology/metabolism
MH  - Time Factors
EDAT- 2007/03/30 09:00
MHDA- 2007/05/10 09:00
CRDT- 2007/03/30 09:00
PHST- 2007/03/30 09:00 [pubmed]
PHST- 2007/05/10 09:00 [medline]
PHST- 2007/03/30 09:00 [entrez]
AID - 232/4/581 [pii]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2007 Apr;232(4):581-9.