PMID- 17394304
OWN - NLM
STAT- MEDLINE
DCOM- 20070604
LR  - 20131121
IS  - 1520-6106 (Print)
IS  - 1520-5207 (Linking)
VI  - 111
IP  - 16
DP  - 2007 Apr 26
TI  - Fluorescence relaxation dynamics of acridine orange in nanosized micellar systems 
      and DNA.
PG  - 4189-99
AB  - In this paper, we report a detailed study of the fluorescence relaxation dynamics 
      of a well-known fluorescent DNA intercalator, acridine orange (AO), in reverse 
      micelles (RM), micelles, and DNA using picosecond resolved fluorescence 
      spectroscopy. Solvation studies of AO in AOT reverse micelles (RM) containing 
      water indicate the locations of AO close to the interface and those in RM 
      containing NaOH; there are two types of AO--one in the nonpolar oil phase and the 
      other at the interface. The bound water at the reverse micellar interface is 
      found to be much more rigid than that at the micellar interface of sodium dodecyl 
      sulfate (SDS) micelles. Dynamic light scattering (DLS) studies allow for the 
      determination of the hydrodynamic radius and the overall tumbling motion of the 
      macromolecules. Wobbling-in-cone data analysis of the temporal fluorescence 
      anisotropy decay allows for determination of restriction on the motion of 
      fluorophores attached to the macromolecules. This model further applied to 
      AO-intercalated genomic DNA and synthetic oligonucleotides within their 
      structural integrity (as confirmed through circular dichroism (CD) studies) shows 
      that AO experiences less restriction in genomic salmon sperm DNA compared with 
      that in synthetic oligonucleotides, and among the oligonucleotides, the ones with 
      AT base pairs are much more rigid. This study would invoke further research on 
      the dynamical nature of AO in restricted environments.
FAU - Shaw, Ajay Kumar
AU  - Shaw AK
AD  - Unit for Nanoscience and Technology, Department of Chemical, Biological, and 
      Macromolecular Sciences, Satyendra Nath Bose National Centre for Basic Sciences, 
      Block JD, Sector III, Salt Lake, Kolkata 700 098, India.
FAU - Pal, Samir Kumar
AU  - Pal SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070330
PL  - United States
TA  - J Phys Chem B
JT  - The journal of physical chemistry. B
JID - 101157530
RN  - 0 (Micelles)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Animals
MH  - Base Sequence
MH  - DNA/*chemistry
MH  - *Micelles
MH  - *Nanoparticles
MH  - Scattering, Radiation
EDAT- 2007/03/31 09:00
MHDA- 2007/06/05 09:00
CRDT- 2007/03/31 09:00
PHST- 2007/03/31 09:00 [pubmed]
PHST- 2007/06/05 09:00 [medline]
PHST- 2007/03/31 09:00 [entrez]
AID - 10.1021/jp067156r [doi]
PST - ppublish
SO  - J Phys Chem B. 2007 Apr 26;111(16):4189-99. doi: 10.1021/jp067156r. Epub 2007 Mar 
      30.