PMID- 17395478
OWN - NLM
STAT- MEDLINE
DCOM- 20070711
LR  - 20181113
IS  - 1044-0305 (Print)
IS  - 1044-0305 (Linking)
VI  - 18
IP  - 6
DP  - 2007 Jun
TI  - An enzyme derivatized polydimethylsiloxane (PDMS) membrane for use in membrane 
      introduction mass spectrometry (MIMS).
PG  - 973-9
AB  - Membrane introduction mass spectrometry (MIMS) provides direct measurement of 
      volatile and semivolatile analytes in condensed and gas-phase samples without 
      sample preparation steps. Although MIMS has numerous advantages that include 
      direct, on-line, real-time analysis with low detection limits, current 
      applications of MIMS are predominantly limited to volatile and semivolatile 
      analytes that permeate hydrophobic membranes (e.g., polydimethylsiloxane; PDMS). 
      We report the first enzyme modified PDMS membrane for use with MIMS. This was 
      achieved by immobilizing Candida rugosa lipase directly onto the surface of 
      oxidized PDMS. These surface immobilized enzymes catalyze ester hydrolysis, 
      releasing an alcohol product at the membrane interface that is readily detected. 
      We have successfully used an enzyme modified membrane for the analysis and 
      quantification of low-volatility and hydrophilic esters. We report the 
      quantification of several carboxylic acid esters in dilute aqueous solutions, 
      including a phthalate monoester carboxylate that is not readily detected by 
      conventional MIMS. This new interface demonstrates the potential for extending 
      the range and versatility of MIMS.
FAU - Creba, A Skye
AU  - Creba AS
AD  - Applied Environmental Research Laboratories, Department of Chemistry, Malaspina 
      University-College, Nanaimo, British Columbia, Canada.
FAU - Weissfloch, Alexandra N E
AU  - Weissfloch AN
FAU - Krogh, Erik T
AU  - Krogh ET
FAU - Gill, Chris G
AU  - Gill CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070328
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Esters)
RN  - 0 (Fungal Proteins)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
RN  - EC 3.1.1.3 (Lip4 protein, Candida rugosa)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Adsorption
MH  - Coated Materials, Biocompatible/*chemistry
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Enzymes, Immobilized/chemistry
MH  - Esters/*analysis
MH  - Fungal Proteins/*chemistry
MH  - Lipase/*chemistry
MH  - Mass Spectrometry/*methods
MH  - *Membranes, Artificial
MH  - Protein Binding
MH  - Silicones/*chemistry
EDAT- 2007/03/31 09:00
MHDA- 2007/07/12 09:00
CRDT- 2007/03/31 09:00
PHST- 2006/12/06 00:00 [received]
PHST- 2007/01/31 00:00 [revised]
PHST- 2007/02/03 00:00 [accepted]
PHST- 2007/03/31 09:00 [pubmed]
PHST- 2007/07/12 09:00 [medline]
PHST- 2007/03/31 09:00 [entrez]
AID - S1044-0305(07)00137-7 [pii]
AID - 10.1016/j.jasms.2007.02.005 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2007 Jun;18(6):973-9. doi: 10.1016/j.jasms.2007.02.005. 
      Epub 2007 Mar 28.