PMID- 17396124
OWN - NLM
STAT- MEDLINE
DCOM- 20070712
LR  - 20161124
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 10
IP  - 5
DP  - 2007 May
TI  - TRPC channels promote cerebellar granule neuron survival.
PG  - 559-67
AB  - Channels formed by the transient receptor potential (TRP) family of proteins have 
      a variety of physiological functions. Here we report that two members of the TRP 
      cation channel (TRPC) subfamily, TRPC3 and 6, protected cerebellar granule 
      neurons (CGNs) against serum deprivation-induced cell death in cultures and 
      promoted CGN survival in rat brain. In CGN cultures, blocking TRPC channels or 
      downregulating TRPC3 or 6 suppressed brain-derived neurotrophic factor 
      (BDNF)-mediated protection, BDNF-triggered intracellular Ca2+ elevation and 
      BDNF-induced CREB activation. By contrast, overexpressing TRPC3 or 6 increased 
      CREB-dependent reporter gene transcription and prevented apoptosis in the neurons 
      deprived of serum, and this protection was blocked by the dominant negative form 
      of CREB. Furthermore, downregulating TRPC3 or 6 induced CGN apoptosis in neonatal 
      rat cerebellum, and this effect was rescued by overexpressing either TRPC3 or 6. 
      Thus, our findings provide in vitro and in vivo evidence that TRPC channels are 
      important in promoting neuronal survival.
FAU - Jia, Yichang
AU  - Jia Y
AD  - Laboratory of Neural Signal Transduction, Institute of Neuroscience, Shanghai 
      Institutes of Biological Sciences, Key Laboratory of Neurobiology, Shanghai 
      200031, China.
FAU - Zhou, Jian
AU  - Zhou J
FAU - Tai, Yilin
AU  - Tai Y
FAU - Wang, Yizheng
AU  - Wang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070401
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Imidazoles)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (TRPC Cation Channels)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.4.22.- (Caspase 3)
RN  - I61V87164A (1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - Nat Neurosci. 2007 May;10(5):537-8. PMID: 17453053
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Calcium/metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Caspase 3/metabolism
MH  - Cell Survival/genetics/physiology
MH  - Cells, Cultured
MH  - Cerebellum/*cytology
MH  - Drug Interactions
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Green Fluorescent Proteins/metabolism
MH  - Imidazoles/pharmacology
MH  - In Situ Nick-End Labeling/methods
MH  - In Vitro Techniques
MH  - Neurons/drug effects/*physiology
MH  - Neuroprotective Agents/pharmacology
MH  - RNA, Small Interfering/pharmacology
MH  - Rats
MH  - TRPC Cation Channels/*physiology
MH  - Transfection/methods
EDAT- 2007/03/31 09:00
MHDA- 2007/07/13 09:00
CRDT- 2007/03/31 09:00
PHST- 2006/10/23 00:00 [received]
PHST- 2007/02/16 00:00 [accepted]
PHST- 2007/03/31 09:00 [pubmed]
PHST- 2007/07/13 09:00 [medline]
PHST- 2007/03/31 09:00 [entrez]
AID - nn1870 [pii]
AID - 10.1038/nn1870 [doi]
PST - ppublish
SO  - Nat Neurosci. 2007 May;10(5):559-67. doi: 10.1038/nn1870. Epub 2007 Apr 1.