PMID- 17397478
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20070402
IS  - 0266-8254 (Print)
IS  - 0266-8254 (Linking)
VI  - 44
IP  - 4
DP  - 2007 Apr
TI  - Eco-physiological characterization of fluorescence in situ hybridization 
      probe-targeted denitrifiers in activated sludge using culture-independent 
      methods.
PG  - 399-405
AB  - AIMS: This study proposes the application of a culture-independent method 
      [fluorescence in situ hybridization (FISH)] and a bioreactor operation control 
      strategy to characterize environmental micro-organisms according to their 
      survival strategies in a mixed suspension culture. Eco-physiological 
      characteristics of two 16S rRNA probe-targeted denitrifiers (DEN581 and DEN124) 
      were investigated against the availability of two resources. METHODS AND RESULTS: 
      Four sequencing batch reactors were operated with manipulation of the sludge 
      retention times to enforce limited and excess availability of two nutrients, 
      namely acetate and nitrite, to the biomass. DEN581 FISH probe-targeted 
      denitrifiers demonstrated dominance when the ratio of either acetate or nitrite 
      to biomass was in excess, while DEN124-targeted organisms dominated when the 
      above were limited. CONCLUSIONS: The study demonstrated that microbial 
      populations in mixed cultures can be selected by changing the substrate 
      availability (Rs) to biomass (X) ratio. The manipulation of the specific resource 
      availability (Rs/X) determined which one of the studied probe-targeted 
      denitrifiers (DEN124 or DEN581) became dominant. Rs/X provides a basis to study 
      the physiology of micro-organisms that cannot be isolated in pure culture from 
      activated sludge. SIGNIFICANCE AND IMPACT OF THE STUDY: The eco-physiological 
      characterization of micro-organisms responsible for biological nutrient removal 
      is anticipated to assist process designers and operators to optimize a specific 
      biological process, such as denitrification.
FAU - Ginige, M P
AU  - Ginige MP
AD  - Advanced Wastewater Management Centre, The University of Queensland, St Lucia, 
      Qld, Australia.
FAU - Carvalho, G
AU  - Carvalho G
FAU - Keller, J
AU  - Keller J
FAU - Blackall, L L
AU  - Blackall LL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Acetates)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Nitrites)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Acetates/metabolism
MH  - Betaproteobacteria/*metabolism
MH  - Biomass
MH  - *Bioreactors
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microbiological Techniques
MH  - Nitrites/metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
EDAT- 2007/04/03 09:00
MHDA- 2007/06/20 09:00
CRDT- 2007/04/03 09:00
PHST- 2007/04/03 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2007/04/03 09:00 [entrez]
AID - LAM2089 [pii]
AID - 10.1111/j.1472-765X.2006.02089.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2007 Apr;44(4):399-405. doi: 
      10.1111/j.1472-765X.2006.02089.x.