PMID- 17397871
OWN - NLM
STAT- MEDLINE
DCOM- 20070627
LR  - 20151119
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 140
IP  - 1
DP  - 2007 Jun 1
TI  - Matrix metalloproteinases and inhibitor in knee synovial fluid as cartilage 
      biomarkers in rabbits: the effect of high-intensity jumping exercise.
PG  - 149-57
AB  - OBJECTIVE: To study the cartilage injury of knee joint in a rabbit model under 
      high-intensity jumping training and to investigate if levels of matrix 
      metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), and tissue 
      inhibitor of matrix metalloproteinase-1 (TIMP-1) in synovial fluid (SF) can be 
      used to predict early sports injury of articular cartilage effectively. MATERIALS 
      AND METHODS: Forty New Zealand white rabbits were divided into two groups 
      randomly: untreated control group (CTRL, n = 8) and jumping training group (TG, n 
      = 32). Concentrations of MMP-1, MMP-3, and TIMP-1 in SF were measured by ELISA 
      assays at 4 and 8 weeks, respectively. Rabbits were euthanized at 4 and 8 weeks, 
      and knee joints were taken out to be examined histologically. Sulfated 
      glycosaminoglycan (GAG) content, thickness of cartilage and subchondral bone, 
      dead cell ratio, and Mankin grades were measured. RESULTS: At 4 weeks, sulfated 
      GAG content, thickness of subchondral bone, and Mankin grades in TG were 
      significantly higher than control. After 8 weeks, the TG cohort had a further 
      increase in the articular cartilage injury. SF levels of MMP-3, TIMP-1, and 
      MMP-3/TIMP-1 in TG were significantly higher than control, and the level of these 
      biomarkers was significantly associated with the severity of the articular 
      cartilage pathology. CONCLUSIONS: Repetitive and high-intensity jumping movement 
      may induce sports injury in the knee joint cartilage. MMP-1, MMP-3, TIMP-1, and 
      MMP-3/TIMP-1 in the SF may accurately predict the severity and pathological 
      characteristic of the joint cartilage injury.
FAU - Qi, Chang
AU  - Qi C
AD  - Military Training Medicine Institute in the 150th Hospital of PLA, Luoyang, Henan 
      Province, China.
FAU - Changlin, Huang
AU  - Changlin H
FAU - Zefeng, Huang
AU  - Zefeng H
LA  - eng
PT  - Journal Article
DEP - 20070330
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Biomarkers)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - *Biomarkers
MH  - Cartilage, Articular/enzymology/injuries
MH  - Knee Injuries/*metabolism/pathology
MH  - Knee Joint/enzymology/pathology
MH  - Matrix Metalloproteinase 1/*metabolism
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Motor Activity/physiology
MH  - Rabbits
MH  - Synovial Fluid/enzymology
MH  - Tissue Inhibitor of Metalloproteinase-1/*metabolism
EDAT- 2007/04/03 09:00
MHDA- 2007/06/28 09:00
CRDT- 2007/04/03 09:00
PHST- 2006/10/04 00:00 [received]
PHST- 2006/12/15 00:00 [revised]
PHST- 2006/12/22 00:00 [accepted]
PHST- 2007/04/03 09:00 [pubmed]
PHST- 2007/06/28 09:00 [medline]
PHST- 2007/04/03 09:00 [entrez]
AID - S0022-4804(06)01181-4 [pii]
AID - 10.1016/j.jss.2006.12.556 [doi]
PST - ppublish
SO  - J Surg Res. 2007 Jun 1;140(1):149-57. doi: 10.1016/j.jss.2006.12.556. Epub 2007 
      Mar 30.