PMID- 17397892
OWN - NLM
STAT- MEDLINE
DCOM- 20070718
LR  - 20181113
IS  - 0042-6822 (Print)
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 364
IP  - 1
DP  - 2007 Jul 20
TI  - Differences in tropism and pH dependence for glycoproteins from the Clade B1 
      arenaviruses: implications for receptor usage and pathogenicity.
PG  - 132-9
AB  - The Clade B lineage of the New World arenaviruses contains four viruses capable 
      of causing severe hemorrhagic fevers in humans. Within this group, the B1 
      sub-lineage contains the pathogenic viruses Junin (JUNV) and Machupo (MACV), as 
      well as the non-pathogenic Tacaribe virus (TCRV). In order to elucidate 
      differences that may determine pathogenicity, we studied the entry pathways 
      directed by the glycoproteins (GPs) from these related B1 viruses, using 
      pseudotyped retroviral vectors and GP1 immunoadhesin constructs. Our data 
      revealed variations in the efficiency with which different cell types could be 
      transduced by B1 vectors, and this correlated with the ability of the 
      immunoadhesins to bind to those cells. Interestingly, the tropism directed by the 
      TCRV GP proved to be distinct from that of JUNV and MACV, in particular on 
      lymphocyte cell lines. In addition, the GPs showed variations in their 
      sensitivity to an inhibitor of endosome acidification, with the TCRV GP again 
      being the outlier. Together these data suggest that more than one entry pathway 
      can be used by these closely related viruses and that the ability to cause human 
      disease may be highly dependent on receptor usage.
FAU - Oldenburg, Jill
AU  - Oldenburg J
AD  - Saban Research Institute of Childrens Hospital Los Angeles, Los Angeles, 
      California 90027, USA.
FAU - Reignier, Therese
AU  - Reignier T
FAU - Flanagan, Meg L
AU  - Flanagan ML
FAU - Hamilton, Genevieve A
AU  - Hamilton GA
FAU - Cannon, Paula M
AU  - Cannon PM
LA  - eng
GR  - U54 AI065359/AI/NIAID NIH HHS/United States
GR  - U54 AI065359-030005/AI/NIAID NIH HHS/United States
GR  - 1U54 AI065359/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070329
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Glycoproteins)
RN  - 0 (Receptors, Virus)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Arenaviridae Infections/virology
MH  - Arenaviruses, New World/classification/genetics/*pathogenicity/*physiology
MH  - CHO Cells
MH  - Cell Line
MH  - Cricetinae
MH  - Cricetulus
MH  - Genetic Vectors
MH  - Glycoproteins/physiology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Junin virus/classification/genetics/pathogenicity/physiology
MH  - Lymphocytes/virology
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Phylogeny
MH  - Receptors, Virus/physiology
MH  - Viral Proteins/physiology
MH  - Virus Internalization
PMC - PMC2743526
MID - NIHMS25518
EDAT- 2007/04/03 09:00
MHDA- 2007/07/19 09:00
PMCR- 2009/09/14
CRDT- 2007/04/03 09:00
PHST- 2006/12/11 00:00 [received]
PHST- 2007/01/29 00:00 [revised]
PHST- 2007/03/05 00:00 [accepted]
PHST- 2007/04/03 09:00 [pubmed]
PHST- 2007/07/19 09:00 [medline]
PHST- 2007/04/03 09:00 [entrez]
PHST- 2009/09/14 00:00 [pmc-release]
AID - S0042-6822(07)00146-8 [pii]
AID - 10.1016/j.virol.2007.03.003 [doi]
PST - ppublish
SO  - Virology. 2007 Jul 20;364(1):132-9. doi: 10.1016/j.virol.2007.03.003. Epub 2007 
      Mar 29.