PMID- 17400203
OWN - NLM
STAT- MEDLINE
DCOM- 20070720
LR  - 20070515
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 381
IP  - 2
DP  - 2007 Jun
TI  - Neovascularization and cardiomyocytes regeneration in acute myocardial infarction 
      after bone marrow stromal cell transplantation: comparison of infarct-relative 
      and noninfarct-relative arterial approaches in swine.
PG  - 114-8
AB  - BACKGROUND: Adult bone marrow stromal cells could differentiate into myogenic 
      endothelial progenitor cells and has been investigated for the potential value in 
      regeneration. Recently, it has been reported that bone marrow cells (BMCs) are 
      able to repair the infracted myocardium by intracoronary transplantation via 
      infarct-related artery in humans. Unfortunately, we cannot open the infarcted 
      artery by traditional reperfusion therapies in some patients. We investigate the 
      hypothesis that BMCs transplantation might get the same effect via 
      noninfarct-relative artery. This alternative approach may have potential 
      application in clinical practice. METHODS: A swine myocardial infarction model 
      was established by distal left anterior descending artery ligation. Bone marrow 
      stromal cells isolated, culture-expanded and labeled with bromodeoxyuridine 
      (BrdU) were used as donor cells. Four weeks after coronary artery ligation, 
      either a graft of 5x10(6) donor cells (n=12) or culture medium (n=6) was infused 
      into infarcted area via infarct-relative artery (left coronary artery, n=6) and 
      noninfarct-relative artery (right coronary artery, n=6). Heart function was 
      evaluated by gate cardiac perfusion imaging before the transplantation and 4 
      weeks after transplantation. The donor cell localization and differentiation were 
      identified by immunohistochemical staining for BrdU and beta-myosin heavy chain 
      (beta-MHC) and angiogenesis was assessed by immunohistochemical staining for 
      alpha-smooth muscle actin (alpha-SMA) and Factor VIII. RESULTS: Gate cardiac 
      perfusion imaging demonstrated that the cardiac function was significantly 
      improved after the stromal cell transplantation via both infarct-relative and 
      noninfarct-relative coronary arteries compared with control group (45.03+/-2.71 
      and 47.78+/-2.64 vs 30.36+/-2.76, P<0.05). Four weeks after transplantation, BrdU 
      and beta-MHC positive cells were detected within the infarct area. Vessel 
      densities in infarct area and infarct border area were increased significantly in 
      both transplantation groups compared to the control group (98.68+/-5.32 and 
      87.49+/-6.04 vs 48.46+/-4.88, P<0.05). CONCLUSIONS: Transplantation of bone 
      marrow stromal cell via both infarct-relative and noninfarct-relative coronary 
      arteries improved heart function in the myocardial infarction animals by 
      stimulating cardiomyocyte regeneration and angiogenesis.
FAU - Yang, Zhi-jian
AU  - Yang ZJ
AD  - Department of Cardiovascular Medicine, The First Affiliated Hospital of Nanjing 
      Medical University, Guangzhou Road 300, Nanjing 210029, Jiangsu Province, China. 
      enzhijia@sohu.com
FAU - Ma, Dong-chao
AU  - Ma DC
FAU - Wang, Wei
AU  - Wang W
FAU - Xu, Shun-lin
AU  - Xu SL
FAU - Zhang, Yu-qing
AU  - Zhang YQ
FAU - Chen, Bo
AU  - Chen B
FAU - Zhou, Fang
AU  - Zhou F
FAU - Zhu, Tie-bing
AU  - Zhu TB
FAU - Wang, Lian-sheng
AU  - Wang LS
FAU - Jia, En-zhi
AU  - Jia EZ
FAU - Zhang, Fu-min
AU  - Zhang FM
FAU - Cao, Ke-jiang
AU  - Cao KJ
FAU - Xu, Zhao-qiang
AU  - Xu ZQ
FAU - Ma, Wen-zhu
AU  - Ma WZ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070223
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Arteries/pathology/*physiology
MH  - *Bone Marrow Transplantation
MH  - Cell Separation
MH  - Coronary Circulation/physiology
MH  - Heart/physiopathology
MH  - Immunohistochemistry
MH  - Myocardial Infarction/*pathology/*therapy
MH  - Myocytes, Cardiac/*physiology
MH  - Neovascularization, Physiologic/*physiology
MH  - Regeneration/*physiology
MH  - Stromal Cells/transplantation
MH  - Swine
EDAT- 2007/04/03 09:00
MHDA- 2007/07/21 09:00
CRDT- 2007/04/03 09:00
PHST- 2006/08/04 00:00 [received]
PHST- 2007/01/11 00:00 [revised]
PHST- 2007/02/08 00:00 [accepted]
PHST- 2007/04/03 09:00 [pubmed]
PHST- 2007/07/21 09:00 [medline]
PHST- 2007/04/03 09:00 [entrez]
AID - S0009-8981(07)00072-1 [pii]
AID - 10.1016/j.cca.2007.02.035 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2007 Jun;381(2):114-8. doi: 10.1016/j.cca.2007.02.035. Epub 2007 
      Feb 23.