PMID- 17403620
OWN - NLM
STAT- MEDLINE
DCOM- 20071107
LR  - 20121115
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 855
IP  - 1
DP  - 2007 Aug
TI  - Simultaneous determination of 13 amphetamine related drugs in human whole blood 
      using an enhanced polymer column and gas chromatography-mass spectrometry.
PG  - 115-20
AB  - Metamphetamine (MA) is one of the most frequently encountered abused drugs in 
      Japan and the Triage immunoassay kit is often used to screen for this drug. 
      However, immunoassay screening also gives positive results with other 
      structurally related compounds, such as 3,4-methylenedioxymethamphetamine (MDMA), 
      3,4-methylenedioxyamphetamine (MDA), p-methoxyamphetamine (PMA), an ephedrine 
      metabolite and beta-phenethylamine (PEA). Therefore, it is important to develop a 
      simple and reliable method which can determine these drugs simultaneously. This 
      paper describes a simple method for simultaneous identification and 
      quantification of 13 amphetamine related drugs in human whole blood. The method 
      consists of a solid phase extraction using a new polar-enhanced Focus column 
      followed by acetylation and gas chromatography-mass spectrometry in the scan 
      mode. Tetradeuterated MA and trideuterated methylephedrine (ME) were used as 
      internal standards. As the Focus column required only simple extraction steps and 
      provided a clean extract, identification of each drug was feasible even at low 
      concentrations. The calibration curves were linear over the concentration range 
      from 50 to 5000 ng/ml for all drugs with correlation coefficients that exceeded 
      0.99. The lower limits of detection of the drugs were 5-50 ng/ml. The absolute 
      recoveries for the drugs were 65-95% and 64-89% at concentrations of 100 and 1000 
      ng/ml, respectively. Accuracy and precision data were satisfactory when using 2 
      internal standards. The applicability of the assay was proven by the analysis of 
      blood samples in forensic cases. This method should be most useful for 
      confirmation of positive immunoassay results for amphetamines and related drugs.
FAU - Kudo, Keiko
AU  - Kudo K
AD  - Department of Forensic Pathology and Sciences, Graduate School of Medical 
      Sciences, Kyushu University, Fukuoka 812-8582, Japan.
FAU - Ishida, Tomomi
AU  - Ishida T
FAU - Hara, Kenji
AU  - Hara K
FAU - Kashimura, Seiichi
AU  - Kashimura S
FAU - Tsuji, Akiko
AU  - Tsuji A
FAU - Ikeda, Noriaki
AU  - Ikeda N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070319
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Amphetamines)
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Phenethylamines)
RN  - 0 (Polymers)
RN  - 327C7L2BXQ (phenethylamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - OVB8F8P39Q (4-methoxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/blood
MH  - Amphetamines/*blood
MH  - Central Nervous System Stimulants/*blood
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Phenethylamines/blood
MH  - Polymers/chemistry
MH  - Reproducibility of Results
EDAT- 2007/04/04 09:00
MHDA- 2007/11/08 09:00
CRDT- 2007/04/04 09:00
PHST- 2006/10/05 00:00 [received]
PHST- 2007/02/28 00:00 [revised]
PHST- 2007/03/01 00:00 [accepted]
PHST- 2007/04/04 09:00 [pubmed]
PHST- 2007/11/08 09:00 [medline]
PHST- 2007/04/04 09:00 [entrez]
AID - S1570-0232(07)00187-0 [pii]
AID - 10.1016/j.jchromb.2007.03.002 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Aug;855(1):115-20. doi: 
      10.1016/j.jchromb.2007.03.002. Epub 2007 Mar 19.