PMID- 17405814
OWN - NLM
STAT- MEDLINE
DCOM- 20071002
LR  - 20211203
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 120
IP  - Pt 9
DP  - 2007 May 1
TI  - Symmetrical localization of extrachromosomally replicating viral genomes on 
      sister chromatids.
PG  - 1529-39
AB  - In eukaryotes, many latent viruses replicate as extrachromosomal molecules, 
      called episomes, and efficiently segregate to daughter cells by noncovalently 
      attaching to mitotic chromosomes. To understand the mechanism governing the 
      processes, we analyzed the detailed subcellular localization of Epstein-Barr 
      virus (EBV) genomes and a viral protein EBNA1, a bridging molecule between viral 
      genomes and cellular chromatin. In the cells that were infected with a 
      recombinant EBV expressing epitope-tagged EBNA1, EBNA1 localized to intranuclear 
      punctate dots, which coincided with the localization of EBV genomes as revealed 
      by fluorescence in situ hybridization (FISH). A significant number of EBNA1 dots 
      were found to localize symmetrically on sister chromatids of mitotic chromosomes. 
      Such symmetrical localization of EBNA1 dots was observed in prematurely condensed 
      G2 chromosomes as well, correlating with the presence of closely spaced double 
      dots of EBNA1 in G2-phase-enriched cells. The EBNA1 double dots were occasionally 
      interconnected by the FISH signals of EBV episomes, exhibiting a dumbbell-like 
      appearance. Thus, we propose that the partitioning of EBNA1 molecules onto sister 
      chromatids during cellular DNA replication underlies the non-stochastic 
      segregation of extrachromosomally replicating viral genomes.
FAU - Kanda, Teru
AU  - Kanda T
AD  - Center for Virus Vector Development, Institute for Genetic Medicine, Hokkaido 
      University, Sapporo 060-0815, Japan. tkanda@igm.hokudai.ac.jp
FAU - Kamiya, Masato
AU  - Kamiya M
FAU - Maruo, Seiji
AU  - Maruo S
FAU - Iwakiri, Dai
AU  - Iwakiri D
FAU - Takada, Kenzo
AU  - Takada K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070403
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Diketopiperazines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Epstein-Barr Virus Nuclear Antigens)
RN  - 0 (Marine Toxins)
RN  - 0 (Oxazoles)
RN  - 0 (Piperazines)
RN  - 0 (Topoisomerase II Inhibitors)
RN  - 0 (centromere protein C)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 21416-68-2 (4,4'-(1,2-dimethyl-1,2-ethanediyl)bis-2,6-piperazinedione)
RN  - 7D07U14TK3 (calyculin A)
RN  - O5GA75RST7 (EBV-encoded nuclear antigen 1)
RN  - X6Q56QN5QC (Hydroxyurea)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Nucleus/chemistry/genetics
MH  - Chromatids/*genetics
MH  - Chromosomal Proteins, Non-Histone/analysis/genetics/metabolism
MH  - DNA Replication/*genetics
MH  - Diketopiperazines
MH  - Enzyme Inhibitors/pharmacology
MH  - Epstein-Barr Virus Nuclear Antigens/analysis/genetics/metabolism
MH  - G2 Phase/genetics
MH  - Gene Dosage
MH  - *Genome, Viral
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Herpesvirus 4, Human/*genetics
MH  - Humans
MH  - Hydroxyurea/pharmacology
MH  - In Situ Hybridization, Fluorescence
MH  - Marine Toxins
MH  - Mitosis/drug effects
MH  - Models, Genetic
MH  - Oxazoles/pharmacology
MH  - Piperazines/pharmacology
MH  - Plasmids/analysis/*genetics
MH  - Topoisomerase II Inhibitors
EDAT- 2007/04/05 09:00
MHDA- 2007/10/03 09:00
CRDT- 2007/04/05 09:00
PHST- 2007/04/05 09:00 [pubmed]
PHST- 2007/10/03 09:00 [medline]
PHST- 2007/04/05 09:00 [entrez]
AID - jcs.03434 [pii]
AID - 10.1242/jcs.03434 [doi]
PST - ppublish
SO  - J Cell Sci. 2007 May 1;120(Pt 9):1529-39. doi: 10.1242/jcs.03434. Epub 2007 Apr 
      3.