PMID- 17406243
OWN - NLM
STAT- MEDLINE
DCOM- 20070621
LR  - 20240104
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 1
IP  - 1
DP  - 2006
TI  - COBRA: combined binary ratio labeling of nucleic-acid probes for multi-color 
      fluorescence in situ hybridization karyotyping.
PG  - 264-75
AB  - Combined binary ratio labeling (COBRA) is designed to increase the multiplicity 
      of fluorescence in situ hybridization (FISH)--i.e., the number of targets that 
      can be distinguished simultaneously. In principle, chemical (ULS), enzymatic 
      (nick translation or random priming) or PCR-based labeling procedures of probes 
      can be used. The method was originally designed to label chromosome-painting 
      probes, but has also been used for probe sets specific for subtelomeric regions. 
      COBRA imaging requires a digital fluorescence microscope equipped for sequential 
      excitation and recording of color images. Staining of all 24 human chromosomes is 
      accomplished with only four fluorochromes, compared with five for methods based 
      on combinatorial labeling. The COBRA procedure takes approximately 6 h laboratory 
      work, 2-3 d incubation and a few hours imaging. The method is routinely applied 
      in research (cultured cells from human or mouse origin) or to support clinical 
      diagnosis, such as postnatal and perinatal genetic testing and in solid tumors.
FAU - Szuhai, Karoly
AU  - Szuhai K
AD  - Department of Molecular Cell Biology, Leiden University Medical Center, PO Box 
      9600 (zone S1-P), 2300 RC Leiden, The Netherlands.
FAU - Tanke, Hans J
AU  - Tanke HJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Nucleic Acid Probes)
SB  - IM
MH  - Animals
MH  - Chromosome Painting/*methods
MH  - Chromosomes, Human
MH  - Fluorescent Dyes/*chemistry
MH  - Humans
MH  - Karyotyping/*methods
MH  - Microscopy, Fluorescence
MH  - Nucleic Acid Probes/*chemistry
MH  - Polymerase Chain Reaction
EDAT- 2007/04/05 09:00
MHDA- 2007/06/22 09:00
CRDT- 2007/04/05 09:00
PHST- 2007/04/05 09:00 [pubmed]
PHST- 2007/06/22 09:00 [medline]
PHST- 2007/04/05 09:00 [entrez]
AID - nprot.2006.41 [pii]
AID - 10.1038/nprot.2006.41 [doi]
PST - ppublish
SO  - Nat Protoc. 2006;1(1):264-75. doi: 10.1038/nprot.2006.41.