PMID- 17406334
OWN - NLM
STAT- MEDLINE
DCOM- 20070921
LR  - 20080324
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 1
IP  - 2
DP  - 2006
TI  - Isolation and purification of afferent lymph dendritic cells that drain the skin 
      of cattle.
PG  - 982-7
AB  - Dendritic cells (DCs) are central to the induction of immune responses and are a 
      pivotal control point that determines the outcome of infectious challenge. 
      Cannulation of afferent lymphatic vessels allows the isolation of large numbers 
      of lymph DCs. First, lymph nodes that are draining the skin are surgically 
      removed (takes approximately 1 h). Over a period of 6-8 weeks, afferent lymphatic 
      vessels re-anastomose with the efferent duct, forming larger 'pseudoafferent' 
      lymphatic vessels that can be surgically cannulated. Surgical cannulation takes 2 
      h to perform; daily maintenance of the catheter requires 30 min. Isolation of 
      lymph cells requires 1 h and an additional 60-180 min to enrich or purify the 
      DCs. The lymph can be harvested for up to 1 month, with relatively constant cell 
      numbers and subset distribution throughout this period. This technique, although 
      technically demanding, facilitates studies of DCs and other cells that traffic in 
      the lymph in both the steady state and following antigenic exposure.
FAU - Hope, Jayne C
AU  - Hope JC
AD  - Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK. 
      jayne.hope@BBSRC.ac.uk
FAU - Howard, Chris J
AU  - Howard CJ
FAU - Prentice, Helen
AU  - Prentice H
FAU - Charleston, Bryan
AU  - Charleston B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Separation/*methods
MH  - Dendritic Cells/*metabolism
MH  - Lymph/*cytology
MH  - Lymphatic System/*cytology/*immunology/surgery
MH  - Skin/cytology/*immunology
MH  - Surgical Procedures, Operative/*methods
EDAT- 2007/04/05 09:00
MHDA- 2007/09/22 09:00
CRDT- 2007/04/05 09:00
PHST- 2007/04/05 09:00 [pubmed]
PHST- 2007/09/22 09:00 [medline]
PHST- 2007/04/05 09:00 [entrez]
AID - nprot.2006.125 [pii]
AID - 10.1038/nprot.2006.125 [doi]
PST - ppublish
SO  - Nat Protoc. 2006;1(2):982-7. doi: 10.1038/nprot.2006.125.