PMID- 17406466
OWN - NLM
STAT- MEDLINE
DCOM- 20071023
LR  - 20080324
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 1
IP  - 5
DP  - 2006
TI  - Collection of lymph-borne dendritic cells in the rat.
PG  - 2263-70
AB  - Dendritic cells (DCs) are crucial in immune induction. Not only do they collect 
      antigens in peripheral tissues, and transport and process them for presentation 
      to lymphocytes in draining lymph nodes, but they also regulate the immune 
      response by modulating T-cell differentiation. Intestinal and hepatic DCs 
      migrating in lymph can be collected from rats under near-physiological 
      conditions. Initially, the mesenteric or celiac lymph nodes are removed from 
      young rats (30 min). The afferent and efferent lymph vessels subsequently heal, 
      permitting DCs to enter the thoracic duct. After at least 6 wk, the duct is 
      cannulated (40 min). Lymph can be collected for up to 48 h. DCs can subsequently 
      be identified, enriched and sorted to high degrees of purity. This two-stage 
      technique generates large numbers of immunologically relevant DCs under 
      near-physiological conditions. Lymph collection requires 2-3 h per animal over 6 
      wk.
FAU - Milling, Simon W F
AU  - Milling SW
AD  - Sir William Dunn School of Pathology, University of Oxford, South Parks Road, 
      Oxford OX1 3RE, UK. simon.milling@path.ox.ac.uk
FAU - Jenkins, Christopher
AU  - Jenkins C
FAU - MacPherson, Gordon
AU  - MacPherson G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
SB  - IM
MH  - Animals
MH  - Catheterization/*methods
MH  - *Dendritic Cells
MH  - Intestines/immunology
MH  - Lymph/*cytology
MH  - Lymph Node Excision
MH  - Male
MH  - Rats
MH  - Thoracic Duct
EDAT- 2007/04/05 09:00
MHDA- 2007/10/24 09:00
CRDT- 2007/04/05 09:00
PHST- 2007/04/05 09:00 [pubmed]
PHST- 2007/10/24 09:00 [medline]
PHST- 2007/04/05 09:00 [entrez]
AID - nprot.2006.315 [pii]
AID - 10.1038/nprot.2006.315 [doi]
PST - ppublish
SO  - Nat Protoc. 2006;1(5):2263-70. doi: 10.1038/nprot.2006.315.