PMID- 1740648
OWN - NLM
STAT- MEDLINE
DCOM- 19920324
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 51
IP  - 1
DP  - 1992 Jan
TI  - Heterogeneity in the mobilization of cytoplasmic calcium by human 
      polymorphonuclear leukocytes in response to fMLP, C5a and IL-8/NAP-1.
PG  - 77-83
AB  - The visible excitation and emission wave-lengths of the recently developed 
      fluorescent Ca2+ indicator fluo-3 permit analysis of the intracellular Ca2+ 
      concentration, [Ca2+]i, in flow cytometry with a 488-nm argon laser. The role of 
      [Ca2+]i in human polymorphonuclear leukocyte heterogeneity was investigated in 
      response to formyl-methionyl-leucyl-phenylalanine (fMLP), C5a, and interleukin 
      8/neutrophil attractant/activation protein 1 (IL-8/NAP-1) by flow cytometry. The 
      [Ca2+]i changes in different subpopulations within a heterogeneous cell 
      suspension were resolved upon stimulation with fMLP. Using an anti-CD16 
      phycoerythrin-conjugated antibody and fluo-3 simultaneously, neutrophils affected 
      and nonaffected in Ca2+ mobilization were distinguished in two patients suffering 
      from glycogen storage disease type 1b. In normal neutrophils, a different time 
      course of Ca2+ mobilization of neutrophil subpopulations immediately after 
      stimulation with fMLP was detected. In addition, after stimulation with a low 
      concentration of IL-8/NAP-1 (10(-10) M) two subsets of neutrophils appeared; one 
      of them showed an increase in [Ca2+]i, while the other did not. These results 
      indicate heterogeneity in the neutrophil signal transduction process involved in 
      Ca2+ mobilization. Therefore, flow cytometric analyses can resolve changes in 
      single-cell [Ca2+]i distribution patterns, which is important for the 
      understanding of [Ca2+]i in neutrophil heterogeneous activation processes.
FAU - Elsner, J
AU  - Elsner J
AD  - Department of Immunobiology, Fraunhofer Institut ITA, Hannover, Germany.
FAU - Kaever, V
AU  - Kaever V
FAU - Emmendorffer, A
AU  - Emmendorffer A
FAU - Breidenbach, T
AU  - Breidenbach T
FAU - Lohmann-Matthes, M L
AU  - Lohmann-Matthes ML
FAU - Roesler, J
AU  - Roesler J
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Aniline Compounds)
RN  - 0 (Interleukin-8)
RN  - 0 (Xanthenes)
RN  - 23D4W0B50Y (Fluo-3)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 80295-54-1 (Complement C5a)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aniline Compounds
MH  - Calcium/*metabolism
MH  - Complement C5a/*pharmacology
MH  - Cytoplasm/drug effects/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-8/*pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/drug effects/*metabolism/physiology
MH  - Spectrometry, Fluorescence
MH  - Time Factors
MH  - Xanthenes
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1002/jlb.51.1.77 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1992 Jan;51(1):77-83. doi: 10.1002/jlb.51.1.77.