PMID- 17406573
OWN - NLM
STAT- MEDLINE
DCOM- 20071105
LR  - 20080324
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 1
IP  - 6
DP  - 2006
TI  - Mutation screening using fluorescence multiplex denaturing gradient gel 
      electrophoresis (FMD): detecting mutations in the BRCA1 gene.
PG  - 3101-10
AB  - Fluorescent multiplex denaturing gradient gel electrophoresis (FMD) is a mutation 
      screening technique designed to detect unknown as well as previously identified 
      mutations. FMD constitutes a recent modification of the standard denaturing 
      gradient gel electrophoresis (DGGE) technique, which combines multiplex PCR 
      amplification of target DNA using fluorescently labeled primers with DGGE 
      separation of the amplicon mixture, allowing immediate identification of sequence 
      variants by wet gel scanning. FMD permits the simultaneous detection of small 
      insertions, deletions and single nucleotide substitutions among multiple DNA 
      fragments (up to 480 fragments) from 96 samples in parallel for each run. It 
      increases output and reduces cost dramatically compared with classical DGGE, 
      without sacrificing sensitivity and accuracy in detecting mutations. This 
      protocol details an accurate, fast, nonradioactive and cost-effective way to 
      screen the BRCA1 gene for mutations with high sensitivity, providing easily 
      interpreted results. It may also be adapted to screen other target genes and/or 
      used in large-scale epidemiological studies.
FAU - Zhang, Shiyu
AU  - Zhang S
AD  - The Centre for Research in Women's Health, University of Toronto, 790 Bay Street, 
      Toronto, Ontario, M5G IN8, Canada.
FAU - Kuperstein, Graciela
AU  - Kuperstein G
FAU - Narod, Steven A
AU  - Narod SA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
RN  - 0 (DNA Primers)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - DNA Mutational Analysis/*methods
MH  - DNA Primers
MH  - Electrophoresis, Gel, Two-Dimensional/*methods
MH  - Fluorescent Dyes
MH  - *Genes, BRCA1
MH  - Humans
MH  - Polymerase Chain Reaction
EDAT- 2007/04/05 09:00
MHDA- 2007/11/06 09:00
CRDT- 2007/04/05 09:00
PHST- 2007/04/05 09:00 [pubmed]
PHST- 2007/11/06 09:00 [medline]
PHST- 2007/04/05 09:00 [entrez]
AID - nprot.2006.445 [pii]
AID - 10.1038/nprot.2006.445 [doi]
PST - ppublish
SO  - Nat Protoc. 2006;1(6):3101-10. doi: 10.1038/nprot.2006.445.