PMID- 1740693
OWN - NLM
STAT- MEDLINE
DCOM- 19920320
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 12
IP  - 2
DP  - 1992 Feb
TI  - Transforming growth factor alpha, but not epidermal growth factor, promotes the 
      survival of sensory neurons in vitro.
PG  - 583-94
AB  - Transforming growth factor alpha (TGF alpha) is a mitogenic polypeptide that is 
      structurally homologous to epidermal growth factor (EGF) and appears to bind to 
      the same receptor in all systems tested previously. In the present study, TGF 
      alpha was found to enhance survival and neurite outgrowth of cultured neonatal 
      rat dorsal root ganglion (DRG) neurons in a dose-dependent manner. This effect 
      was observed with TGF alpha concentrations as low as 17.8 pM. By contrast, EGF at 
      concentrations up to 83 nM was ineffective. Moreover, EGF did not antagonize the 
      TGF alpha survival-promoting effect unless present in large excess (500-fold the 
      concentration for which TGF alpha is effective); even in this case, only partial 
      antagonism was achieved. Survival of neurons from nodose, trigeminal, and 
      sympathetic ganglia was not increased by TGF alpha. Both a subpopulation of DRG 
      neurons and of macrophages in the cultures bound iodinated TGF alpha. This 
      binding was inhibited by excess unlabeled TGF alpha but not EGF. Our data are 
      consistent with the possibilities that the actions of TGF alpha on DRG neurons 
      occur indirectly via unidentified neurotrophic molecules other than NGF as well 
      as directly on the neurons themselves. Thus, TGF alpha, in contrast to EGF, may 
      act as a survival or maintenance factor for a subset of rat sensory neurons. 
      Mediation of this neurotrophic effect appears to occur via a new form of TGF 
      alpha receptor.
FAU - Chalazonitis, A
AU  - Chalazonitis A
AD  - Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York.
FAU - Kessler, J A
AU  - Kessler JA
FAU - Twardzik, D R
AU  - Twardzik DR
FAU - Morrison, R S
AU  - Morrison RS
LA  - eng
GR  - NS20013/NS/NINDS NIH HHS/United States
GR  - NS26125/NS/NINDS NIH HHS/United States
GR  - NS26766/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Transforming Growth Factor alpha)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid
MH  - Dose-Response Relationship, Drug
MH  - Epidermal Growth Factor/metabolism/*pharmacology
MH  - ErbB Receptors/metabolism
MH  - Ganglia, Spinal/*cytology
MH  - Humans
MH  - Kinetics
MH  - Neurons, Afferent/drug effects/*physiology
MH  - Rats
MH  - Transforming Growth Factor alpha/isolation & purification/*pharmacology
PMC - PMC6575603
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
PMCR- 1992/08/01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
PHST- 1992/08/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.12-02-00583.1992 [doi]
PST - ppublish
SO  - J Neurosci. 1992 Feb;12(2):583-94. doi: 10.1523/JNEUROSCI.12-02-00583.1992.