PMID- 17407068
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20070404
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 24
IP  - 2
DP  - 2007 Apr
TI  - [The clinical application of whole chromosome painting probes in preimplantation 
      genetic diagnosis for translocation carriers].
PG  - 140-3
AB  - OBJECTIVE: To make preimplantation genetic diagnosis (PGD) for female 
      translocation carriers by analyzing first polar bodies (1PBs) with whole 
      chromosome painting probe (WCP). METHODS: WCP was used in fluorescence in situ 
      hybridization (FISH) analysis of 1PBs for four female Robertsonian carriers 
      presented for PGD with 45 XX, der(13;14)(q10;q10) karyotype. All the patients 
      underwent ovarian stimulation and during 6 h after oocyte retrieval 1PBs were 
      biopsied and WCP were used in FISH. On day 3 after fertilization embryos 
      diagnosed as normal or balanced were transferred. RESULTS: A total of 61 oocytes 
      were collected in 4 PGD cycles. Of the 54 matured oocytes, 50 were biopsied and 
      45 were fixed successfully. Results were obtained in 40 1PBs. Overall, 74.1% 
      (40/54) oocytes were diagnosed. The fertilization rate and good embryo rate were 
      64.8% (35/54) and 65.7% (23/35) respectively. Two clinical pregnancies were 
      obtained. One patient delivered a normal female baby with karyotype 46, XX in 
      June 2006. For another patient, the fetus spontaneously aborted at 9th week of 
      pregnancy with karyotype of 45, X confirmed by amniotic villus diagnosis. 
      CONCLUSION: WCP can differentiate normal, balanced and unbalanced oocytes 
      accurately and can be used as an efficient PGD method for female carriers of 
      translocation.
FAU - Ren, Xiu-lian
AU  - Ren XL
AD  - Reproductive Medical Center, First Affiliated Hospital, SUN Yat-sen University, 
      Guangzhou, Guangdong, 510080 PR China.
FAU - Xu, Yan-wen
AU  - Xu YW
FAU - Zhuang, Guang-lun
AU  - Zhuang GL
FAU - Zhou, Can-quan
AU  - Zhou CQ
FAU - Liu, Ying
AU  - Liu Y
FAU - Ou, Jian-ping
AU  - Ou JP
FAU - Li, Sui-ping
AU  - Li SP
LA  - chi
PT  - Case Reports
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Chromosome Painting/*methods
MH  - Female
MH  - Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Oocytes/metabolism
MH  - Pregnancy
MH  - Preimplantation Diagnosis/*methods
MH  - Translocation, Genetic/*genetics
EDAT- 2007/04/05 09:00
MHDA- 2009/03/28 09:00
CRDT- 2007/04/05 09:00
PHST- 2007/04/05 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
PHST- 2007/04/05 09:00 [entrez]
AID - 940624030 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Apr;24(2):140-3.