PMID- 17408061
OWN - NLM
STAT- MEDLINE
DCOM- 20070504
LR  - 20171116
IS  - 0300-0605 (Print)
IS  - 0300-0605 (Linking)
VI  - 35
IP  - 1
DP  - 2007 Jan-Feb
TI  - Nifedipine, a calcium-channel blocker, inhibits advanced glycation 
      end-product-induced expression of monocyte chemoattractant protein-1 in human 
      cultured mesangial cells.
PG  - 107-12
AB  - The interaction between advanced glycation end-products (AGEs) and their 
      receptors mediates the progressive alteration in renal architecture and loss of 
      renal function in diabetic nephropathy. This study investigated whether 
      nifedipine, a widely used anti-hypertensive drug, suppresses expression of 
      monocyte chemoattractant protein-1 (MCP-1), a chemokine that mediates the 
      recruitment of monocytes to inflammatory sites, in AGE-exposed human cultured 
      mesangial cells. Cells were treated with 100 microg/ml AGE-bovine serum albumin 
      (BSA) or non-glycated BSA in the presence or absence of 1 microM nifedipine or 50 
      nM diphenylene iodonium, an inhibitor of reduced nicotinamide-adenine 
      dinucleotide phosphate oxidase, for 4 or 24 h. Expression of MCP-1 mRNA was 
      measured using a semi-quantitative reverse transcription-polymerase chain 
      reaction; MCP-1 protein production was measured using an enzyme-linked 
      immunosorbent assay. AGEs significantly increased both MCP-1 mRNA expression and 
      protein production in mesangial cells; this increase was blocked by both 
      nifedipine and diphenylene iodonium. These results suggest that nifedipine could 
      play a protective role against early diabetic nephropathy by suppressing MCP-1 
      overexpression via blockade of AGE signalling in mesangial cells.
FAU - Matsui, T
AU  - Matsui T
AD  - Department of Internal Medicine, Kurume University School of Medicine, Kurume, 
      Japan.
FAU - Yamagishi, S
AU  - Yamagishi S
FAU - Nakamura, K
AU  - Nakamura K
FAU - Inoue, H
AU  - Inoue H
FAU - Takeuchi, M
AU  - Takeuchi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Int Med Res
JT  - The Journal of international medical research
JID - 0346411
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (RNA, Messenger)
RN  - I9ZF7L6G2L (Nifedipine)
SB  - IM
MH  - Calcium Channel Blockers/*pharmacology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*antagonists & inhibitors/biosynthesis/genetics
MH  - Glomerular Mesangium/cytology/*drug effects
MH  - Glycation End Products, Advanced/antagonists & inhibitors/*physiology
MH  - Humans
MH  - Nifedipine/*pharmacology
MH  - RNA, Messenger/metabolism
EDAT- 2007/04/06 09:00
MHDA- 2007/05/05 09:00
CRDT- 2007/04/06 09:00
PHST- 2007/04/06 09:00 [pubmed]
PHST- 2007/05/05 09:00 [medline]
PHST- 2007/04/06 09:00 [entrez]
AID - 10.1177/147323000703500111 [doi]
PST - ppublish
SO  - J Int Med Res. 2007 Jan-Feb;35(1):107-12. doi: 10.1177/147323000703500111.