PMID- 17408790 OWN - NLM STAT- MEDLINE DCOM- 20070719 LR - 20220129 IS - 0167-7012 (Print) IS - 0167-7012 (Linking) VI - 69 IP - 3 DP - 2007 Jun TI - Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization. PG - 523-8 AB - An updated dataset of in silico specificities for 54 previously published 16S rRNA-targeted oligonucleotides was assembled to provide guidance for reliable fluorescence in situ hybridization (FISH) analysis of sulfate-reducing bacteria. Additionally, six new FISH probes were developed for major deltaproteobacterial taxa, including a probe trio targeting most Deltaproteobacteria and Gemmatimonadetes. FAU - Lucker, Sebastian AU - Lucker S AD - Department of Microbial Ecology, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. FAU - Steger, Doris AU - Steger D FAU - Kjeldsen, Kasper Urup AU - Kjeldsen KU FAU - MacGregor, Barbara J AU - MacGregor BJ FAU - Wagner, Michael AU - Wagner M FAU - Loy, Alexander AU - Loy A LA - eng GR - P 18836/FWF_/Austrian Science Fund FWF/Austria PT - Evaluation Study PT - Journal Article DEP - 20070228 PL - Netherlands TA - J Microbiol Methods JT - Journal of microbiological methods JID - 8306883 RN - 0 (Oligonucleotide Probes) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Sulfates) SB - IM MH - Databases, Nucleic Acid MH - Deltaproteobacteria/genetics/isolation & purification MH - In Situ Hybridization, Fluorescence/*methods MH - *Oligonucleotide Probes MH - RNA, Ribosomal, 16S/*genetics MH - Sensitivity and Specificity MH - Species Specificity MH - Sulfates/*metabolism MH - Sulfur-Reducing Bacteria/genetics/*isolation & purification EDAT- 2007/04/06 09:00 MHDA- 2007/07/20 09:00 CRDT- 2007/04/06 09:00 PHST- 2006/12/22 00:00 [received] PHST- 2007/01/31 00:00 [revised] PHST- 2007/02/20 00:00 [accepted] PHST- 2007/04/06 09:00 [pubmed] PHST- 2007/07/20 09:00 [medline] PHST- 2007/04/06 09:00 [entrez] AID - S0167-7012(07)00064-4 [pii] AID - 10.1016/j.mimet.2007.02.009 [doi] PST - ppublish SO - J Microbiol Methods. 2007 Jun;69(3):523-8. doi: 10.1016/j.mimet.2007.02.009. Epub 2007 Feb 28.