PMID- 17409065 OWN - NLM STAT- MEDLINE DCOM- 20070913 LR - 20181113 IS - 0016-6731 (Print) IS - 0016-6731 (Linking) VI - 176 IP - 1 DP - 2007 May TI - Integrative mapping of Gossypium hirsutum L. by meiotic fluorescent in situ hybridization of a tandemly repetitive sequence (B77). PG - 115-23 AB - We determined the relative positions of the tandem-repeat molecular cytogenetic marker B77, translocation breakpoints, and telosome arms in Gossypium hirsutum cytogenetic stocks by fluorescence in situ hybridization (FISH) analysis of meiotic quadrivalents in 16 single and 2 double translocation heterozygotes and five monotelodisomics. Results delimited the B77 FISH locus to the right arm of the D-subgenome chromosome 14 (14R) and the short arm (14sh), respectively. By equating 14R with 14sh and 14L (left) with 14Lo (long), the findings established a unified nomenclature for the arms of chromosome 14. Previously reported chromosome 14 arm locations were confirmed for four of the five translocations involving chromosome 14, namely NT1L-14L (2780), NT2R-14R (2B-1), NT14L-23R (2777), and NT14R-24R (2781), whereas the location of breakpoint T6L-14L was not confirmed and was reassigned to arm 14R. When used as a probe on Southern blots, the B77 signal was associated with a terminus of the D-subgenome RFLP linkage group (LG) D04 by linkage analysis of an interspecific F(2) population, now known to be chromosome 20. However, additional codominant DNA marker information in the affected region excluded the B77 polymorphism detected by Southern blot hybridization from chromosome 20 and, indeed, from the remainder of the genome. FAU - Ji, Yuanfu AU - Ji Y AD - Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843, USA. FAU - Zhao, Xinping AU - Zhao X FAU - Paterson, Andrew H AU - Paterson AH FAU - Price, H James AU - Price HJ FAU - Stelly, David M AU - Stelly DM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20070403 PL - United States TA - Genetics JT - Genetics JID - 0374636 RN - 0 (Genetic Markers) SB - IM MH - Chromosome Breakage MH - *Chromosome Mapping MH - Chromosomes, Plant/genetics MH - Genetic Markers MH - Gossypium/cytology/*genetics MH - Heterozygote MH - *In Situ Hybridization, Fluorescence MH - Meiosis/*genetics MH - Pachytene Stage MH - Tandem Repeat Sequences/*genetics PMC - PMC1893065 EDAT- 2007/04/06 09:00 MHDA- 2007/09/14 09:00 PMCR- 2007/08/01 CRDT- 2007/04/06 09:00 PHST- 2007/04/06 09:00 [pubmed] PHST- 2007/09/14 09:00 [medline] PHST- 2007/04/06 09:00 [entrez] PHST- 2007/08/01 00:00 [pmc-release] AID - genetics.107.071738 [pii] AID - gen1761115 [pii] AID - 10.1534/genetics.107.071738 [doi] PST - ppublish SO - Genetics. 2007 May;176(1):115-23. doi: 10.1534/genetics.107.071738. Epub 2007 Apr 3.