PMID- 17409643
OWN - NLM
STAT- MEDLINE
DCOM- 20070718
LR  - 20190606
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 69
IP  - 3
DP  - 2007 Mar
TI  - The significance of p53 and retinoblastoma pathways in canine hemangiosarcoma.
PG  - 271-8
AB  - To investigate whether inactivation of the p53 and retinoblastoma (Rb) protein 
      pathways contributes to the development of canine hemangiosarcoma (HSA), we 
      examined immunohistochemically the expression of p53, Rb, phosphorylated Rb 
      (phospho-Rb), p16, and cyclin D1 in 39 spontaneous canine HSAs and 10 
      hemangiomas. In addition, mutations in the p53 gene were analyzed by polymerase 
      chain reaction (PCR)-single-stranded conformation polymorphism and PCR direct 
      sequencing; furthermore, we quantified cyclin D1 mRNA by semiquantitative 
      real-time reverse transcription-PCR. Positive immunoreactivity for p53 was 
      observed in 17.9% of HSAs. However, mutations were not detected in these cases. 
      The labeling indices for Rb, phospho-Rb, and cyclin D1 were markedly higher in 
      all HSAs than in hemangiomas. Of the 7 cases with cyclin D1-positive 
      immunoreactivity, 4 overexpressed cyclin D1 mRNA (to a level more than 10-fold 
      higher than that of GAPDH mRNA). The p16 protein was clearly detected in all 
      hemangiomas; however, 82% of the neoplastic cells in HSA showed a loss of or low 
      immunoreactivity. These results suggest that alteration of the p16-cyclin D1-Rb 
      pathway, rather than the p53 pathway, may be associated with the pathogenesis of 
      canine HSA.
FAU - Yonemaru, Kayoko
AU  - Yonemaru K
AD  - Laboratoriy of Veterinary Pathology, Department of Veterinary Medicine, Faculty 
      of Applied Biological Sciences, Gifu University, Japan.
FAU - Sakai, Hiroki
AU  - Sakai H
FAU - Murakami, Mami
AU  - Murakami M
FAU - Kodama, Atsushi
AU  - Kodama A
FAU - Mori, Takashi
AU  - Mori T
FAU - Yanai, Tokuma
AU  - Yanai T
FAU - Maruo, Kohji
AU  - Maruo K
FAU - Masegi, Toshiaki
AU  - Masegi T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (DNA Primers)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cyclin D1/genetics/metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p16/metabolism
MH  - DNA Primers
MH  - Dog Diseases/*metabolism
MH  - Dogs
MH  - Hemangiosarcoma/metabolism/*veterinary
MH  - Immunohistochemistry/veterinary
MH  - Molecular Sequence Data
MH  - Polymorphism, Single-Stranded Conformational
MH  - Retinoblastoma Protein/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Sequence Analysis, DNA/veterinary
MH  - Signal Transduction/*physiology
MH  - Tumor Suppressor Protein p53/genetics/*metabolism
EDAT- 2007/04/06 09:00
MHDA- 2007/07/19 09:00
CRDT- 2007/04/06 09:00
PHST- 2007/04/06 09:00 [pubmed]
PHST- 2007/07/19 09:00 [medline]
PHST- 2007/04/06 09:00 [entrez]
AID - JST.JSTAGE/jvms/69.271 [pii]
AID - 10.1292/jvms.69.271 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2007 Mar;69(3):271-8. doi: 10.1292/jvms.69.271.