PMID- 17412370
OWN - NLM
STAT- MEDLINE
DCOM- 20070720
LR  - 20161124
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 620
IP  - 1-2
DP  - 2007 Jul 1
TI  - Chromosomal aberrations by fluorescence in situ hybridization (FISH)--Biomarker 
      of exposure to carcinogenic PAHs.
PG  - 62-70
AB  - The fluorescence in situ hybridization (FISH) technique with whole chromosome 
      painting for chromosomes #1 and #4 was used to study the impact of air pollution 
      containing higher concentrations of carcinogenic polycyclic aromatic hydrocarbons 
      (c-PAHs) in three European cities, Prague (Czech Republic), Kosice (Slovakia) and 
      Sofia (Bulgaria). In each site were followed an exposed group, who were police 
      officers or bus drivers who work usually through busy streets for at least 8h, 
      and a reference group, who spent more than 90% of their daily time indoors. In 
      Prague, a significant increase was observed in percentage of aberrant cells (% 
      AB.C.) in the police officers compared to the reference group (0.33+/-0.25 versus 
      0.24+/-0.18, p<0.05). In Kosice, the exposed group differed from reference in the 
      endpoints F(G)/100 1.52+/-1.18 versus 1.12+/-1.30, p<0.05; % AB.C. 0.30+/-0.19 
      versus 0.21+/-0.20, p<0.05; t/1000 3.91+/-3.18 versus 2.84+/-3.10, p<0.05. In 
      Sofia were followed two exposed groups: police officers and bus drivers. All FISH 
      endpoints were significantly higher in police officers compared to reference 
      group (F(G)/100 1.60+/-0.99 versus 0.82+/-0.79, p<0.01; % AB.C. 0.25+/-0.14 
      versus 0.13+/-0.13, p<0.01; t/1000 4.19+/-2.65 versus 2.13+/-2.05, p<0.05; rcp 
      1.46+/-1.07 versus 0.70+/-0.76, p<0.05). In bus drivers compared to reference 
      there was an increase in % AB.C. (0.25+/-0.18 versus 0.13+/-0.13, p<0.05). This 
      is the first study when FISH method was used to analyze the impact of 
      environmental air pollution. According to the original hypothesis it is expected 
      that the most important group of chemicals responsible for the biological 
      activity of air pollution represent c-PAHs.
FAU - Beskid, Olena
AU  - Beskid O
AD  - Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, 
      v.v.i., and Health Institute of Central Bohemia, Prague, Czech Republic.
FAU - Binkova, Blanka
AU  - Binkova B
FAU - Dusek, Zdik
AU  - Dusek Z
FAU - Rossner, Pavel
AU  - Rossner P
FAU - Solansky, Ivo
AU  - Solansky I
FAU - Kalina, Ivan
AU  - Kalina I
FAU - Zidzik, Jozef
AU  - Zidzik J
FAU - Popov, Todor A
AU  - Popov TA
FAU - Farmer, Peter B
AU  - Farmer PB
FAU - Sram, Radim J
AU  - Sram RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070303
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Air Pollutants)
RN  - 0 (Biomarkers)
RN  - 0 (Carcinogens, Environmental)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
SB  - IM
MH  - Adult
MH  - Air Pollutants/*toxicity
MH  - Biomarkers
MH  - Carcinogens, Environmental/*toxicity
MH  - *Chromosome Aberrations
MH  - Environmental Monitoring
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Occupational Exposure
MH  - Police
MH  - Polycyclic Aromatic Hydrocarbons/*toxicity
EDAT- 2007/04/07 09:00
MHDA- 2007/07/21 09:00
CRDT- 2007/04/07 09:00
PHST- 2007/04/07 09:00 [pubmed]
PHST- 2007/07/21 09:00 [medline]
PHST- 2007/04/07 09:00 [entrez]
AID - S0027-5107(07)00104-2 [pii]
AID - 10.1016/j.mrfmmm.2007.02.023 [doi]
PST - ppublish
SO  - Mutat Res. 2007 Jul 1;620(1-2):62-70. doi: 10.1016/j.mrfmmm.2007.02.023. Epub 
      2007 Mar 3.