PMID- 17414225
OWN - NLM
STAT- MEDLINE
DCOM- 20070508
LR  - 20220408
IS  - 0160-2446 (Print)
IS  - 0160-2446 (Linking)
VI  - 49
IP  - 3
DP  - 2007 Mar
TI  - Anti-atherogenic effects of a mixture of ascorbic acid, lysine, proline, 
      arginine, cysteine, and green tea phenolics in human aortic smooth muscle cells.
PG  - 140-5
AB  - Certain drastic behavioral modifications by arterial wall smooth muscle cells 
      (SMC) have been considered key steps in the formation of atherosclerotic lesions: 
      massive migration of SMC from the media to the intima layer of the vessel, 
      dedifferentiation of SMC to proliferating phenotype, and increased secretion of 
      inflammatory cytokines as a response to inflammatory stimuli. We investigated the 
      anti-atherogenic effects of naturally occurring compounds (ascorbic acid, green 
      tea extract, lysine, proline, arginine, and N-acetyl cysteine) using the model of 
      cultured aortic SMC. Cell growth was measured by DNA synthesis, cell invasiveness 
      was measured through Matrigel, matrix metalloproteinase-2 (MMP-2) secretion was 
      measured by zymography, and SMC secretion of monocyte chemoattractant protein-1 
      (MCP-1) and interleukin-6 (IL-6) was measured by immunochemistry. Fetal bovine 
      serum-stimulated SMC growth was inhibited by the nutrient mixture (NM) with 85% 
      inhibition at 100 microg/mL. A corresponding concentration of epigallocatechin 
      gallate (EGCG; 15 microM), the most active tea phenolic, produced a significant 
      effect but one lower than NM. NM inhibited aortic SMC Matrigel invasion in a 
      dose-dependent manner and significantly decreased MMP-2 expression. Stimulation 
      of SMC with tumor necrosis factor-alpha significantly increased production and 
      secretion of such mediators of inflammation as IL-6 and MCP-1; addition of 100 
      microg/mL NM inhibited secretion of MCP-1 and IL-6 by 65% and 47%, respectively. 
      These data suggest that the NM of ascorbic acid, tea phenolics, and selected 
      amino acids has potential in blocking the development of atherosclerotic lesions 
      by inhibiting atherogenic responses of vascular SMC to pathologic stimuli and 
      warrants in vivo studies.
FAU - Ivanov, Vadim
AU  - Ivanov V
AD  - Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, California 95050, 
      USA.
FAU - Roomi, M Waheed
AU  - Roomi MW
FAU - Kalinovsky, T
AU  - Kalinovsky T
FAU - Niedzwiecki, Aleksandra
AU  - Niedzwiecki A
FAU - Rath, Matthias
AU  - Rath M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cardiovasc Pharmacol
JT  - Journal of cardiovascular pharmacology
JID - 7902492
RN  - 0 (Amino Acids)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 0 (Tea)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Amino Acids/*pharmacology
MH  - Animals
MH  - Aorta/cytology/drug effects
MH  - Ascorbic Acid/*pharmacology
MH  - Atherosclerosis/*drug therapy/prevention & control
MH  - Catechin/analogs & derivatives/pharmacology
MH  - Cattle
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Immunochemistry
MH  - Interleukin-6/metabolism
MH  - Matrix Metalloproteinase 2/drug effects/metabolism
MH  - Myocytes, Smooth Muscle
MH  - Phenols/isolation & purification/pharmacology
MH  - Plant Extracts/*pharmacology
MH  - Tea/*chemistry
EDAT- 2007/04/07 09:00
MHDA- 2007/05/09 09:00
CRDT- 2007/04/07 09:00
PHST- 2007/04/07 09:00 [pubmed]
PHST- 2007/05/09 09:00 [medline]
PHST- 2007/04/07 09:00 [entrez]
AID - 00005344-200703000-00004 [pii]
AID - 10.1097/FJC.0b013e3180308489 [doi]
PST - ppublish
SO  - J Cardiovasc Pharmacol. 2007 Mar;49(3):140-5. doi: 10.1097/FJC.0b013e3180308489.