PMID- 17418186
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20191210
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 589
IP  - 2
DP  - 2007 Apr 25
TI  - Coupling continuous ultrasound-assisted extraction with ultrasonic probe, 
      solid-phase extraction and high-performance liquid chromatography for the 
      determination of sodium Danshensu and four tanshinones in Salvia miltiorrhiza 
      bunge.
PG  - 231-8
AB  - A dynamic continuous ultrasound-assisted extraction with high intensity 
      ultrasonic probe (CUAE-HIUP) combined with solid-phase extraction (SPE) for 
      preconcentration and clean-up of the extract prior to high-performance liquid 
      chromatography (HPLC) determination of the main biological active ingredients, 
      sodium Danshensu and four tanshinones (dihydrotanshione I, tanshinone I, 
      cryptotanshinone and tanshinone IIA) from root of Salvia miltiorrhiza bunge has 
      been developed. An experimental design (Plackett-Burman design, 2(6)x3/16) was 
      used to optimize the CUAE-HIUP procedure and the SPE step. Detection limits of 
      1.2-4.2 x 10(-6) mg mL(-1) were achieved with the preconcentration efficiency of 
      more than 10-folds by the SPE procedure. The repeatability and within-laboratory 
      reproducibility of the whole process, expressed as relative standard deviations 
      (RSDs), were 1.5-3.6%, 1.6-3.1%, respectively. As compared with the conventional 
      extraction techniques such as room temperature extraction (24 h), Soxhlet (4 h) 
      and even microwave-assisted extraction (2 min), CUAE-HIUP achieved the highest 
      extraction yield (98.9%) and the least amount (10 mL) of solvent compared with 
      the other techniques (16 mL) in only 3 min. The method was successfully applied 
      to the determination of the five biological active ingredients in root of S. 
      miltiorrhiza bunge and Danshen-containing pharmaceutical formulations.
FAU - Yang, Qiao
AU  - Yang Q
AD  - State Key Laboratory of Virology, College of Life Sciences, Wuhan University, 
      Wuhan, China.
FAU - Zhang, Xiao-ling
AU  - Zhang XL
FAU - Li, Xi-yin
AU  - Li XY
FAU - Tang, Wei-kun
AU  - Tang WK
FAU - Zhang, Jun-xiang
AU  - Zhang JX
FAU - Fang, Cheng-xiang
AU  - Fang CX
FAU - Zheng, Cong-yi
AU  - Zheng CY
LA  - eng
PT  - Journal Article
DEP - 20070312
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Abietanes)
RN  - 0 (Lactates)
RN  - 0 (Phenanthrenes)
RN  - 03UUH3J385 (tanshinone)
RN  - 4GF33A5PAJ (3,4-dihydroxyphenyllactic acid)
SB  - IM
MH  - Abietanes
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Lactates/*analysis
MH  - Phenanthrenes/*analysis
MH  - Salvia miltiorrhiza/*chemistry
MH  - Sensitivity and Specificity
MH  - *Ultrasonics
EDAT- 2007/04/10 09:00
MHDA- 2007/06/20 09:00
CRDT- 2007/04/10 09:00
PHST- 2007/01/13 00:00 [received]
PHST- 2007/02/28 00:00 [revised]
PHST- 2007/03/02 00:00 [accepted]
PHST- 2007/04/10 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2007/04/10 09:00 [entrez]
AID - S0003-2670(07)00489-8 [pii]
AID - 10.1016/j.aca.2007.03.005 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2007 Apr 25;589(2):231-8. doi: 10.1016/j.aca.2007.03.005. Epub 
      2007 Mar 12.