PMID- 17418594
OWN - NLM
STAT- MEDLINE
DCOM- 20070723
LR  - 20131121
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 31
IP  - 8
DP  - 2007 Aug
TI  - Transforming growth factor beta-1 enhances cytotoxic effect of doxorubicin in 
      human lung adenocarcinoma cells of A549 line.
PG  - 851-5
AB  - Transforming growth factor beta-1 (TGFbeta-1) is a regulator of cell 
      proliferation, differentiation and apoptosis. Doxorubicin (adriamycin), an 
      anthracycline drug causing double-strand DNA breaks, is widely used in anticancer 
      chemotherapy. Here we demonstrated that TGFbeta-1 enhanced cytotoxic 
      (proapoptotic) action of doxorubicin towards cultured human lung carcinoma A549 
      cells. Western-blot analysis and immunocytochemistry were used to show that 
      doxorubicin induced PARP degradation in A549 cells, and TGFbeta-1 enhanced that 
      action of the drug. The obtained results suggest a possibility of biomodulating 
      effect of TGFbeta-1 on tumor cell treatment with doxorubicin.
FAU - Filyak, Yevhen
AU  - Filyak Y
AD  - Department of Regulation of Cell Proliferation, Institute of Cell Biology NAS of 
      Ukraine, Drahomanova Street 14-16, 79005 Lviv, Ukraine.
FAU - Filyak, Oksana
AU  - Filyak O
FAU - Stoika, Rostyslav
AU  - Stoika R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070228
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Transforming Growth Factor beta1)
RN  - 80168379AG (Doxorubicin)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
MH  - Adenocarcinoma/diet therapy
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Doxorubicin/*therapeutic use
MH  - Drug Synergism
MH  - Humans
MH  - Immunohistochemistry
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Transforming Growth Factor beta1/*physiology
EDAT- 2007/04/10 09:00
MHDA- 2007/07/24 09:00
CRDT- 2007/04/10 09:00
PHST- 2006/10/13 00:00 [received]
PHST- 2007/01/02 00:00 [revised]
PHST- 2007/02/19 00:00 [accepted]
PHST- 2007/04/10 09:00 [pubmed]
PHST- 2007/07/24 09:00 [medline]
PHST- 2007/04/10 09:00 [entrez]
AID - S1065-6995(07)00055-8 [pii]
AID - 10.1016/j.cellbi.2007.02.008 [doi]
PST - ppublish
SO  - Cell Biol Int. 2007 Aug;31(8):851-5. doi: 10.1016/j.cellbi.2007.02.008. Epub 2007 
      Feb 28.