PMID- 17418595
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20221207
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 31
IP  - 9
DP  - 2007 Sep
TI  - Cisplatin cytotoxicity is enhanced with zoledronic acid in A549 lung cancer cell 
      line: preliminary results of an in vitro study.
PG  - 1069-71
AB  - We tested whether zoledronic acid, a biphosphonate with proposed apoptotic 
      activity, augmented the cytotoxicity of cisplatin and/or gemcitabine in A549 lung 
      cancer cell line. This cell line was subjected to different concentrations of the 
      above chemotherapeutic agents and zoledronic acid. Cytotoxicity was assessed by 
      the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide) assay. 
      Particularly, zoledronic acid in 100 micromolar (microM) concentration augmented 
      the cytotoxicity by cisplatin 1microg/ml from 25% to 70% (Z=3.22, P=0.0072). A 
      significant portion of cells underwent apoptosis with or without zoledronic acid, 
      but more so with the combination treatment as assessed by an Annexin V-FITC 
      apoptosis detection kit. However, 100microM zoledronic acid showed 50% 
      cytotoxicity on its own, but failed to improve cytotoxicity by Gemcitabine. Thus, 
      we show for the first time in a lung cancer cell line that zoledronic acid bears 
      cytotoxic potential on its own and in conjunction with cisplatin. The clinical 
      potential of this finding should be further studied.
FAU - Ozturk, O H
AU  - Ozturk OH
AD  - Akdeniz University, Department of Biochemistry, Antalya, Turkey.
FAU - Bozcuk, H
AU  - Bozcuk H
FAU - Burgucu, D
AU  - Burgucu D
FAU - Ekinci, D
AU  - Ekinci D
FAU - Ozdogan, M
AU  - Ozdogan M
FAU - Akca, S
AU  - Akca S
FAU - Yildiz, M
AU  - Yildiz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070225
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Bone Density Conservation Agents)
RN  - 0 (Diphosphonates)
RN  - 0 (Imidazoles)
RN  - 0W860991D6 (Deoxycytidine)
RN  - 6XC1PAD3KF (Zoledronic Acid)
RN  - Q20Q21Q62J (Cisplatin)
RN  - 0 (Gemcitabine)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Bone Density Conservation Agents/pharmacology
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cisplatin/*toxicity
MH  - Deoxycytidine/analogs & derivatives/pharmacology
MH  - Diphosphonates/*pharmacology
MH  - *Drug Screening Assays, Antitumor
MH  - Drug Synergism
MH  - Humans
MH  - Imidazoles/*pharmacology
MH  - Lung Neoplasms/*pathology
MH  - Zoledronic Acid
MH  - Gemcitabine
EDAT- 2007/04/10 09:00
MHDA- 2007/08/02 09:00
CRDT- 2007/04/10 09:00
PHST- 2006/07/17 00:00 [received]
PHST- 2007/02/01 00:00 [revised]
PHST- 2007/02/18 00:00 [accepted]
PHST- 2007/04/10 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/04/10 09:00 [entrez]
AID - S1065-6995(07)00058-3 [pii]
AID - 10.1016/j.cellbi.2007.02.004 [doi]
PST - ppublish
SO  - Cell Biol Int. 2007 Sep;31(9):1069-71. doi: 10.1016/j.cellbi.2007.02.004. Epub 
      2007 Feb 25.