PMID- 1741910
OWN - NLM
STAT- MEDLINE
DCOM- 19920110
LR  - 20220318
IS  - 0934-8840 (Print)
IS  - 0934-8840 (Linking)
VI  - 275
IP  - 3
DP  - 1991 Aug
TI  - Coagglutination test for rapid detection of Salmonella enterotoxin.
PG  - 303-11
AB  - The Staphylococcal Coagglutination (CoA) test using Staphylococcus aureus (Cowan 
      type-1) cells coated with antiserum against purified Salmonella enterotoxin was 
      standardized and carried out to screen Salmonella serotypes for their 
      enterotoxigenicity. Of the 101 cell-free culture supernatants from an equal 
      number of Salmonella strains belonging to 15 different serotypes tested, 76 were 
      found to be enterotoxigenic. All of the 24 strains which gave positive reactions 
      in the rabbit ligated ileal loop test were also positive with this test. As 
      little as 7.5 ng of purified Salmonella enterotoxin could be detected by the CoA 
      test. The presence of enterotoxin in polymyxin B extract of cells and cell-free 
      preparation of a S. typhimurium strain was detected as early as after 1.5 and 3.0 
      h of incubation, respectively. Besides the test standardized with the antiserum 
      to purified V. cholerae toxin (CT) could detect purified toxin in a quantity as 
      little as 5.0 ng and also enterotoxigenicity in an E. coli strain (LT+). The 
      usefulness of the test as a routine, rapid and economic one for the detection of 
      enterotoxin of Salmonella, Vibrio cholerae and E. coli is discussed.
FAU - Rahman, H
AU  - Rahman H
AD  - Department of Microbiology and Public Health, College of Veterinary Sciences, 
      G.B. Pant University of Agric. and Tech., Dist. Nainital (U.P.), India.
FAU - Singh, V B
AU  - Singh VB
FAU - Sharma, V D
AU  - Sharma VD
FAU - Harne, S D
AU  - Harne SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Zentralbl Bakteriol
JT  - Zentralblatt fur Bakteriologie : international journal of medical microbiology
JID - 9203851
RN  - 0 (Bacterial Toxins)
RN  - 0 (Endotoxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Immune Sera)
RN  - 0 (salmonella toxin)
SB  - IM
MH  - *Agglutination Tests
MH  - Animals
MH  - Bacterial Toxins/*analysis/immunology/isolation & purification
MH  - Biological Assay
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Endotoxins
MH  - Enterotoxins/*analysis/immunology/isolation & purification
MH  - Immune Sera/immunology
MH  - Immunoelectrophoresis
MH  - Rabbits
MH  - *Salmonella
EDAT- 1991/08/01 00:00
MHDA- 2000/05/11 00:00
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 2000/05/11 00:00 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - 10.1016/s0934-8840(11)80293-8 [doi]
PST - ppublish
SO  - Zentralbl Bakteriol. 1991 Aug;275(3):303-11. doi: 10.1016/s0934-8840(11)80293-8.