PMID- 17420227
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20071219
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 25
IP  - 4
DP  - 2007 Apr
TI  - Genome-wide differential gene expression profiling of human bone marrow stromal 
      cells.
PG  - 994-1002
AB  - Bone marrow stromal cells (BMSCs) reside in bone marrow and provide a lifelong 
      source of new cells for various connective tissues. Although human BMSCs are 
      regarded as highly suitable for the development of cell therapeutics and 
      regenerative medicine, the molecular factors and the networks of signaling 
      pathways responsible for their biological properties are as yet unclear. To gain 
      a comprehensive understanding of human BMSCs at the transcriptional level, we 
      have performed DNA microarray-based, genome-wide differential gene expression 
      analysis with the use of peripheral blood-derived mononuclear cells (MNCs) as a 
      baseline. The resulting molecular profile of BMSCs was revealed to share no 
      meaningful overlap with those of other human stem cell types, suggesting that the 
      cells might express a unique set of genes for their stemness. By contrast, the 
      distinct molecular signature, consisting of 92 different genes whose expression 
      strengths are at least 50-fold higher in BMSCs compared with MNCs, was shown to 
      encompass largely a gene subset of umbilical cord blood-derived adherent cells, 
      suggesting that adherent cells derived from bone marrow and umbilical cord blood 
      may be defined by a common set of genes, regardless of their origin. 
      Intriguingly, a large number of these genes, particularly ones for extracellular 
      matrix products, coincide with normal or tumor endothelium-specific markers. 
      Taken together, our results here provide a BMSC-specific genetic catalog that may 
      facilitate future studies on molecular mechanisms governing core properties of 
      these cells.
FAU - Jeong, Ju Ah
AU  - Jeong JA
AD  - Biotherapeutic Division, GenExel-Sein Inc., Daejon, Korea.
FAU - Ko, Kyung-Min
AU  - Ko KM
FAU - Bae, Sohyun
AU  - Bae S
FAU - Jeon, Choon-Ju
AU  - Jeon CJ
FAU - Koh, Gou Young
AU  - Koh GY
FAU - Kim, Hoeon
AU  - Kim H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Adipogenesis
MH  - Antigens, CD/analysis
MH  - Bone Marrow Cells/*cytology/*physiology
MH  - Cell Differentiation
MH  - Chondrogenesis
MH  - Female
MH  - *Gene Expression Profiling
MH  - *Genome, Human
MH  - Humans
MH  - Leukocytes, Mononuclear/cytology/physiology
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - Osteogenesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cells/cytology/physiology
MH  - Stromal Cells/cytology/*physiology
MH  - Transcription, Genetic
EDAT- 2007/04/11 09:00
MHDA- 2007/08/02 09:00
CRDT- 2007/04/11 09:00
PHST- 2007/04/11 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/04/11 09:00 [entrez]
AID - 25/4/994 [pii]
AID - 10.1634/stemcells.2006-0604 [doi]
PST - ppublish
SO  - Stem Cells. 2007 Apr;25(4):994-1002. doi: 10.1634/stemcells.2006-0604.