PMID- 17421000
OWN - NLM
STAT- MEDLINE
DCOM- 20070614
LR  - 20191210
IS  - 1042-0533 (Print)
IS  - 1042-0533 (Linking)
VI  - 19
IP  - 3
DP  - 2007 May-Jun
TI  - Enzyme immunoassay for total immunoglobulin E in dried blood spots.
PG  - 440-2
AB  - Elevated circulating levels of total immunoglobulin E (IgE) are associated with 
      both allergic disease and repeated macro-parasitic infections. Population-based 
      research on IgE has been limited by the logistical constraints associated with 
      obtaining and processing venipuncture blood samples. In this short report, we 
      present an enzyme immunoassay protocol for quantifying circulating total IgE 
      levels in capillary whole blood, collected from a finger prick and dried on 
      filter paper. The assay demonstrated acceptable levels of accuracy, precision, 
      and reliability. IgE remained stable at room temperature for only 2-4 days and 
      degraded rapidly at higher temperatures suggesting that samples should be 
      refrigerated or frozen within 1-2 days of collection. It is hoped that the 
      relative ease of blood spot collection will expand opportunities for 
      population-based research on IgE.
CI  - Copyright 2007 Wiley-Liss, Inc.
FAU - Tanner, Susan
AU  - Tanner S
AD  - Department of Anthropology, University of Georgia, Athens, Georgia 30602, USA. 
      stanner1@uga.edu
FAU - McDade, Thomas W
AU  - McDade TW
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Validation Study
PL  - United States
TA  - Am J Hum Biol
JT  - American journal of human biology : the official journal of the Human Biology 
      Council
JID - 8915029
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Blood Specimen Collection/methods/*standards
MH  - Clinical Protocols
MH  - Humans
MH  - *Immunoenzyme Techniques
MH  - Immunoglobulin E/analysis/*blood
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2007/04/11 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/04/11 09:00
PHST- 2007/04/11 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/04/11 09:00 [entrez]
AID - 10.1002/ajhb.20635 [doi]
PST - ppublish
SO  - Am J Hum Biol. 2007 May-Jun;19(3):440-2. doi: 10.1002/ajhb.20635.