PMID- 17421026
OWN - NLM
STAT- MEDLINE
DCOM- 20071128
LR  - 20131121
IS  - 1552-4922 (Print)
IS  - 1552-4922 (Linking)
VI  - 71
IP  - 9
DP  - 2007 Sep
TI  - Re-evaluating acridine orange for rapid flow cytometric enumeration of 
      parasitemia in malaria-infected rodents.
PG  - 662-7
AB  - Methods facilitating research in malaria are of pivotal relevance. Flow cytometry 
      offers the possibility of rapid enumeration of parasitemia. It relies on staining 
      the parasite DNA to distinguish between infected and non-infected red blood cell 
      (RBC) populations. Unfortunately, in rodents abundant reticulocyte RNA interferes 
      with the application of the method. This results in time-consuming sample 
      preparation protocols that offer no clear advantage over microscopic counting. We 
      re-evaluated the use of the DNA/RNA discriminating vital fluorochrome acridine 
      orange (AO) for rapid flow cytometric enumeration of parasitemia in rodents. 
      Whole blood from rodents infected with Plasmodium berghei and Plasmodium yoelii 
      was stained with AO and analyzed by flow cytometer. A newly developed two-channel 
      (FL1/FL3) detection method was compared with conventional one-channel (FL1) 
      detection and microscopic counting. The new AO two-channel detection method 
      clearly discriminated between infected and non-infected RBC populations. It 
      showed to be linear above parasitemias of 0.3%. Sample processing time amounted 
      to approximately 5 min. It is shown that AO can be used for rapid, precise, and 
      accurate enumeration of parasitemia in rodents. Due to its ease of handling the 
      method might find widespread application in malaria research.
CI  - Copyright (c) 2007 International Society for Analytical Cytology.
FAU - Bhakdi, Sebastian Chakrit
AU  - Bhakdi SC
AD  - Division of Instruments for Research, Office for Research and Development, 
      Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Sratongno, Panudda
AU  - Sratongno P
FAU - Chimma, Pattamawan
AU  - Chimma P
FAU - Rungruang, Thanaporn
AU  - Rungruang T
FAU - Chuncharunee, Aporn
AU  - Chuncharunee A
FAU - Neumann, Hartmut P H
AU  - Neumann HP
FAU - Malasit, Prida
AU  - Malasit P
FAU - Pattanapanyasat, Kovit
AU  - Pattanapanyasat K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
RN  - 0 (Fluorescent Dyes)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
CIN - Cytometry A. 2007 Sep;71(9):643-5. PMID: 17712779
MH  - *Acridine Orange
MH  - Animals
MH  - Cricetinae
MH  - Erythrocytes/*parasitology
MH  - Flow Cytometry/*methods
MH  - *Fluorescent Dyes
MH  - Malaria/diagnosis/*parasitology
MH  - Mesocricetus
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Parasitemia/parasitology
MH  - Plasmodium berghei/*isolation & purification/parasitology
MH  - Plasmodium yoelii/*isolation & purification/parasitology
MH  - Staining and Labeling
EDAT- 2007/04/11 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/04/11 09:00
PHST- 2007/04/11 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/04/11 09:00 [entrez]
AID - 10.1002/cyto.a.20406 [doi]
PST - ppublish
SO  - Cytometry A. 2007 Sep;71(9):662-7. doi: 10.1002/cyto.a.20406.