PMID- 17425424
OWN - NLM
STAT- MEDLINE
DCOM- 20070511
LR  - 20210308
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 20
IP  - 1
DP  - 2007 Spring
TI  - Restricted and selective tropism of a Venezuelan equine encephalitis 
      virus-derived replicon vector for human dendritic cells.
PG  - 88-104
AB  - Dendritic cells (DCs) consist of heterogeneous phenotypic populations and have 
      diverse immunostimulatory functions dependent on both lineage and functional 
      phenotype, but as exceptionally potent antigen-presenting cells, they are targets 
      for generating effective antigen-specific immune responses. A promising replicon 
      particle vector derived from Venezuelan equine encephalitis virus (VEE) has been 
      reported to transduce murine footpad DCs. However, the receptive DC subset, the 
      degree of restriction for this tropism, and the extent of conservation between 
      rodents and humans have not been well characterized. Using fresh peripheral blood 
      DCs, mononuclear cells, monocyte-derived macrophages, and monocyte-derived DCs, 
      our results demonstrate conservation of VEE replicon particle (VRP) tropism for 
      DCs between humans and rodents. We observed that a subset of immature myeloid DCs 
      is the target population, and that VRP-transduced immature DCs retain intact 
      functional capacity, for example, the ability to resist the cytopathic effects of 
      VRP transduction and the capacity to acquire the mature phenotype. These studies 
      support the demonstration of selective VRP tropism for human DCs and provide 
      further insight into the biology of the VRP vector, its parent virus, and human 
      DCs.
FAU - Nishimoto, Kevin P
AU  - Nishimoto KP
AD  - Molecular Biology and Biochemistry, School of Medicine, School of Biological 
      Sciences, University of California, Irvine, Irvine, California 92697, USA.
FAU - Laust, Amanda K
AU  - Laust AK
FAU - Wang, Kehui
AU  - Wang K
FAU - Kamrud, Kurt I
AU  - Kamrud KI
FAU - Hubby, Bolyn
AU  - Hubby B
FAU - Smith, Jonathan F
AU  - Smith JF
FAU - Nelson, Edward L
AU  - Nelson EL
LA  - eng
GR  - P30 CA062203/CA/NCI NIH HHS/United States
GR  - P41 EB015890/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
SB  - IM
MH  - Dendritic Cells/physiology/*virology
MH  - Encephalitis Virus, Venezuelan Equine/*genetics/immunology
MH  - Genetic Vectors/*genetics
MH  - Humans
MH  - *Replicon
MH  - Transduction, Genetic
MH  - Tropism
EDAT- 2007/04/12 09:00
MHDA- 2007/05/12 09:00
CRDT- 2007/04/12 09:00
PHST- 2007/04/12 09:00 [pubmed]
PHST- 2007/05/12 09:00 [medline]
PHST- 2007/04/12 09:00 [entrez]
AID - 10.1089/vim.2006.0090 [doi]
PST - ppublish
SO  - Viral Immunol. 2007 Spring;20(1):88-104. doi: 10.1089/vim.2006.0090.