PMID- 17426764
OWN - NLM
STAT- MEDLINE
DCOM- 20070501
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 49
IP  - 11
DP  - 2006 Nov
TI  - Tracing B-genome chromatin in Brassica napus x B. juncea interspecific progeny.
PG  - 1490-7
AB  - We used polymerase chain reaction (PCR) and fluorescence in situ hybridization 
      (FISH) techniques to demonstrate the presence of Brassica B-genome chromosomes 
      and putative B-genome introgressions in B. napus x B. juncea interspecific 
      progeny. The B-genome--specific repeat sequence pBNBH35 was used to generate PCR 
      products and FISH probes. The highest frequencies of viable progeny were obtained 
      when B. napus was the maternal parent of the interspecific hybrid and the first 
      backcross. B-genome--positive PCR assays were found in 34/51 fertile F2 progeny 
      (67%), which was more than double the proportion found in fertile BC(1) progeny. 
      Four B-genome--positive F(2)-derived families and 1 BC(1)-derived family were 
      fixed or segregating for B. juncea morphology in the F(4) and BC(1)S(2), 
      respectively, but in only 2 of these families did B. juncea-type plants exhibit 
      B. juncea chromosome count (2n = 36) and typical B-genome FISH signals on 16 
      chromosomes. The remaining B. juncea-type plants had B. napus chromosome count 
      (2n = 38) and no B-genome FISH signals, except for 1 exceptional F(4)-derived 
      line that exhibited isolated and weak B-genome FISH signals on 11 chromosomes and 
      typical A-genome FISH signals. B. juncea morphology was associated with 
      B-genome--positive PCR signals but not necessarily with 16 intact B-genome 
      chromosomes as detected by FISH. B-genome chromosomes tend to be eliminated 
      during selfing or backcrossing after crossing B. juncea with B. napus, and 
      selection of lines containing B-genome chromatin during early generations would 
      be promoted by use of this B-genome repetitive marker.
FAU - Schelfhout, C J
AU  - Schelfhout CJ
AD  - School of Plant Biology, Faculty of Natural and Agricultural Sciences, The 
      University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia.
FAU - Snowdon, R
AU  - Snowdon R
FAU - Cowling, W A
AU  - Cowling WA
FAU - Wroth, J M
AU  - Wroth JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Chromatin)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Brassica/*genetics
MH  - Brassica napus/genetics
MH  - Chromatin/*genetics
MH  - *Genetic Markers
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction/methods
EDAT- 2007/04/12 09:00
MHDA- 2007/05/02 09:00
CRDT- 2007/04/12 09:00
PHST- 2007/04/12 09:00 [pubmed]
PHST- 2007/05/02 09:00 [medline]
PHST- 2007/04/12 09:00 [entrez]
AID - g06-103 [pii]
AID - 10.1139/g06-103 [doi]
PST - ppublish
SO  - Genome. 2006 Nov;49(11):1490-7. doi: 10.1139/g06-103.