PMID- 17426785
OWN - NLM
STAT- MEDLINE
DCOM- 20080327
LR  - 20191110
IS  - 1213-8118 (Print)
IS  - 1213-8118 (Linking)
VI  - 150
IP  - 2
DP  - 2006 Nov
TI  - Our experiences with measurement of new potential biomarkers in the diagnosis of 
      latent forms of myocardial ischemia.
PG  - 239-44
AB  - BACKGROUND: The need for a laboratory marker of myocardial ischemia has been 
      alluded to for at least the last decade. AIM: The aim of this study was to 
      evaluate the diagnostic importance of the myosin light chain-1 (MLC-1), clusterin 
      and Reg-Ialpha in patients with suspected myocardial ischemia. METHODS: A group 
      of 176 at high-risk for myocardial ischemia subjects was evaluated and divided 
      into two subgroups using myocardial SPECT (Single Photon Emission Computed 
      Tomography) - individuals with and without signs of myocardial ischemia. 
      Laboratory markers in venous blood were repeatedly examined in all subjects: a) 
      immediately prior to SPECT: C-reactive protein, Haemoglobin, Hematocrite, 
      Lactate, MLC-1, Clusterin, Reg-Ialpha b) at subjective maximum: Hb, Htc, lactate, 
      MLC-1, Clusterin, Reg-Ialpha c) 30 min after stress levels reached their peak: 
      MLC-1, Clusterin, Reg-Ialpha and d) 60 min after peak stress levels: MLC-1, 
      Clusterin, Reg-Ialpha. RESULTS: Patients were divided into subgroups according to 
      their positive and negative SPECT results (positive: n = 37; negative: n = 139). 
      MLC-1 values were different for all 4 blood collections. An increase in MLC-1 > 
      2.2 mg/l showed 64 % sensitivity and 88 % specificity for the diagnosed presence 
      of myocardial ischemia (AUC 0.81; LR+ 5.9; PPV+ 68 % and NPV- 87 %). There was no 
      significant difference between the groups in terms of Clusterin and Reg-Ialpha 
      for any of the sampling periods. CONCLUSIONS: High diagnostic efficacy of 
      detectable MLC-1 was shown for the diagnosis of latent myocardial ischemia. 
      Measurement of serum Clusterin or Reg-Ialpha did not sufficient for the diagnosis 
      of latent myocardial ischemia.
FAU - Stejskal, David
AU  - Stejskal D
AD  - Department of Laboratory Medicine and Internal Department, Sternberk Hospital, 
      Czech Republic. david.stejskal@nemstbk.cz
FAU - Lacnak, Borek
AU  - Lacnak B
FAU - Karpisek, Michal
AU  - Karpisek M
FAU - Kaminek, Milan
AU  - Kaminek M
LA  - eng
PT  - Journal Article
PL  - Czech Republic
TA  - Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub
JT  - Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, 
      Czechoslovakia
JID - 101140142
RN  - 0 (Biomarkers)
RN  - 0 (Clusterin)
RN  - 0 (Lithostathine)
RN  - 0 (Myosin Light Chains)
RN  - 0 (REG1A protein, human)
RN  - 0 (myosin light chain I)
SB  - IM
MH  - Biomarkers/*blood
MH  - Clusterin/blood
MH  - Female
MH  - Humans
MH  - Lithostathine/blood
MH  - Male
MH  - Myocardial Ischemia/*diagnosis
MH  - Myosin Light Chains/blood
MH  - Sensitivity and Specificity
MH  - Tomography, Emission-Computed, Single-Photon
EDAT- 2007/04/12 09:00
MHDA- 2008/03/28 09:00
CRDT- 2007/04/12 09:00
PHST- 2007/04/12 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2007/04/12 09:00 [entrez]
AID - 10.5507/bp.2006.034 [doi]
PST - ppublish
SO  - Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006 Nov;150(2):239-44. doi: 
      10.5507/bp.2006.034.