PMID- 17426931
OWN - NLM
STAT- MEDLINE
DCOM- 20070925
LR  - 20220716
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 300
IP  - 1-2
DP  - 2007 Jun
TI  - Glyceraldehyde-3-phosphate dehydrogenase exerts different biologic activities in 
      apoptotic and proliferating hepatocytes according to its subcellular 
      localization.
PG  - 19-28
AB  - Recent evidences indicate new roles for the glycolytic protein 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in essential mammalian cell 
      processes, such as apoptosis and proliferation. To clarify the involvement of 
      this protein in growth and programmed cell death in the liver, cell models of 
      hepatocytes in culture were used to study GAPDH expression, localization and 
      enzymatic activity in hepatocyte proliferation and apoptosis. GAPDH expression in 
      cell compartments was studied by Western blot. Nuclear expression of GAPDH 
      increased in apoptosis, and cytoplasmic expression was elevated in apoptosis and 
      proliferation. Subcellular localization was determined by GAPDH immunostaining 
      and confocal microscopic analysis. Quiescent and proliferating hepatocytes showed 
      cytoplasmic GAPDH, while apoptotic cells showed cytoplasmic but also some nuclear 
      staining. The glycolytic activity of GAPDH was studied in nuclear and cytoplasmic 
      cell compartments. GAPDH enzymatic activity increased in the nucleus of apoptotic 
      cells and in cytoplasms of apoptotic and proliferating hepatocytes. Our 
      observations indicate that during hepatocyte apoptosis GAPDH translocates to the 
      nucleus, maintaining in part its dehydrogenase activity, and suggest that this 
      translocation may play a role in programmed hepatocyte death. GAPDH 
      over-expression and the increased enzymatic activity in proliferating cells, with 
      preservation of its cytoplasmic localization, would occur in response to the 
      elevated energy requirements of dividing hepatocytes. In conclusion, GAPDH plays 
      different roles or biological activities in proliferating and apoptotic 
      hepatocytes, according to its subcellular localization.
FAU - Barbini, Luciana
AU  - Barbini L
AD  - Departamento de Fisiologia, Facultad de Veterinaria, Universidad de Santiago de 
      Compostela, Campus Universitario, 27002 Lugo, Spain. lbarbini@ffyb.uba.ar
FAU - Rodriguez, Joaquin
AU  - Rodriguez J
FAU - Dominguez, Fernando
AU  - Dominguez F
FAU - Vega, Felix
AU  - Vega F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070411
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Transforming Growth Factor beta1)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - *Apoptosis/drug effects
MH  - Cell Nucleus/drug effects/enzymology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cycloheximide/pharmacology
MH  - DNA Fragmentation/drug effects
MH  - Enzyme Induction/drug effects
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
MH  - Glycolysis/drug effects
MH  - Hepatocyte Growth Factor/pharmacology
MH  - Hepatocytes/*cytology/drug effects/*enzymology
MH  - Male
MH  - Protein Transport/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Subcellular Fractions/drug effects/enzymology
MH  - Transforming Growth Factor beta1/pharmacology
EDAT- 2007/04/12 09:00
MHDA- 2007/09/26 09:00
CRDT- 2007/04/12 09:00
PHST- 2006/07/06 00:00 [received]
PHST- 2006/09/28 00:00 [accepted]
PHST- 2007/04/12 09:00 [pubmed]
PHST- 2007/09/26 09:00 [medline]
PHST- 2007/04/12 09:00 [entrez]
AID - 10.1007/s11010-006-9341-1 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2007 Jun;300(1-2):19-28. doi: 10.1007/s11010-006-9341-1. Epub 
      2007 Apr 11.