PMID- 17427270
OWN - NLM
STAT- MEDLINE
DCOM- 20070529
LR  - 20131121
IS  - 0161-6412 (Print)
IS  - 0161-6412 (Linking)
VI  - 29
IP  - 1
DP  - 2007 Jan
TI  - Localization of bone marrow stromal cells in injured spinal cord treated by 
      intravenous route depends on the hemorrhagic lesions in traumatized spinal 
      tissues.
PG  - 21-6
AB  - OBJECTIVES: Bone marrow stromal cells (BMSCs) have been reported to improve 
      movement deficit in adult rats with spinal cord injury (SCI). The purpose of this 
      study is to determine the distribution of BMSCs in the spinal cord lesion of the 
      contusion model of SCI. METHODS: Laminectomy was carried out at L1 vertebra level 
      and SCI was carried out using the weight drop method. BMSCs were isolated from 
      adult rats, labeled with bromodeoxyuridine (BrdU) and administered intravenously 
      to the rats 1 week after SCI, which were killed after 4 weeks. The non-treated 
      animals were used as negative control, which showed cavitations of the spinal 
      cord after 5 weeks of SCI. Rats in another group were killed immediately and used 
      to study the hemorrhagic lesions. The volume densities (Vv) of the hemorrhage and 
      cavitation were the highest at the site of direct trauma. RESULTS: The numerical 
      densities of the transmigrated cells per area (Nat) were as follows: 0.3 +/- 0.2, 
      3.9 +/- 0.4, 5.4 +/- 0.4, 8.4 +/- 0.5, 5.5 +/- 0.3, 3.6 +/- 0.3 and 0.4 +/- 0.2 
      at the end and the middle of the thoracic vertebra 13 (T13), the region between 
      T13 and the first lumbar vertebra, the middle of L1, the region between L1 and 
      L2, and the middle and the end of L2 vertebra, respectively. The distribution of 
      Nat at the above regions was a Gaussian model. The volume densities of hemorrhage 
      in the spinal cord taken from the above regions showed that hemorrhage with the 
      highest volume density occurred at the impact site and the volume density 
      declined as the samples taken were more distant from the impact site. DISCUSSION: 
      The migration of BMSCs in the injured region depends on the amount of the 
      hemorrhage and damage to blood vessels of the spinal cord.
FAU - Khalatbary, Ali Reza
AU  - Khalatbary AR
AD  - Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modarres 
      University, Tehran, Iran.
FAU - Tiraihi, Taki
AU  - Tiraihi T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*methods/trends
MH  - Bromodeoxyuridine
MH  - Cell Movement/physiology
MH  - Female
MH  - Hemorrhage/*physiopathology
MH  - Injections, Intravenous/methods
MH  - Lumbar Vertebrae
MH  - Normal Distribution
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/blood supply/*physiopathology
MH  - Spinal Cord Injuries/pathology/physiopathology/*therapy
MH  - Stromal Cells/*physiology/*transplantation
MH  - Thoracic Vertebrae
MH  - Treatment Outcome
EDAT- 2007/04/12 09:00
MHDA- 2007/05/30 09:00
CRDT- 2007/04/12 09:00
PHST- 2007/04/12 09:00 [pubmed]
PHST- 2007/05/30 09:00 [medline]
PHST- 2007/04/12 09:00 [entrez]
AID - 10.1179/016164107X165642 [doi]
PST - ppublish
SO  - Neurol Res. 2007 Jan;29(1):21-6. doi: 10.1179/016164107X165642.