PMID- 17428474
OWN - NLM
STAT- MEDLINE
DCOM- 20070710
LR  - 20161019
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 205
IP  - 2
DP  - 2007 Jun
TI  - Electrical stimulation promotes sensory neuron regeneration and growth-associated 
      gene expression.
PG  - 347-59
AB  - Brief electrical stimulation enhances the regenerative ability of axotomized 
      motor [Nix, W.A., Hopf, H.C., 1983. Electrical stimulation of regenerating nerve 
      and its effect on motor recovery. Brain Res. 272, 21-25; Al-Majed, A.A., Neumann, 
      C.M., Brushart, T.M., Gordon, T., 2000. Brief electrical stimulation promotes the 
      speed and accuracy of motor axonal regeneration. J. Neurosci. 20, 2602-2608] and 
      sensory [Brushart, T.M., Jari, R., Verge, V., Rohde, C., Gordon, T., 2005. 
      Electrical stimulation restores the specificity of sensory axon regeneration. 
      Exp. Neurol. 194, 221-229] neurons. Here we examined the parameter of duration of 
      stimulation on regenerative capacity, including the intrinsic growth programs, of 
      sensory neurons. The effect of 20 Hz continuous electrical stimulation on the 
      number of DRG sensory neurons that regenerate their axons was evaluated following 
      transection and surgical repair of the femoral nerve trunk. Stimulation was 
      applied proximal to the repair site for 1 h, 3 h, 1 day, 7 days or 14 days at the 
      time of nerve repair. Following a 21-day regeneration period, DRG neurons that 
      regenerated axons into the muscle and cutaneous sensory nerve branches were 
      retrogradely identified. Stimulation of 1 h led to a significant increase in DRG 
      neurons regenerating into cutaneous and muscle branches when compared to 0 h 
      (sham) stimulation or longer periods of stimulation. Stimulation for 1 h also 
      significantly increased the numbers of neurons that regenerated axons beyond the 
      repair site 4 days after lesion and was correlated with a significant increase in 
      expression of growth-associated protein 43 (GAP-43) mRNA in the regenerating 
      neurons at 2 days post-repair. An additional indicator of heightened plasticity 
      following 1 h stimulation was elevated expression of brain-derived neurotrophic 
      factor (BDNF). The effect of brief stimulation on enhancing sensory and 
      motoneuron regeneration holds promise for inducing improved peripheral nerve 
      repair in the clinical setting.
FAU - Geremia, Nicole M
AU  - Geremia NM
AD  - Department of Anatomy and Cell Biology, Cameco MS/Neuroscience Research Center 
      University of Saskatchewan, Saskatoon City Hospital, Saskatchewan, Canada.
FAU - Gordon, Tessa
AU  - Gordon T
FAU - Brushart, Thomas M
AU  - Brushart TM
FAU - Al-Majed, Abdulhakeem A
AU  - Al-Majed AA
FAU - Verge, Valerie M K
AU  - Verge VM
LA  - eng
GR  - R01 NS034484/NS/NINDS NIH HHS/United States
GR  - R01 NS034484-07/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070221
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Activating Transcription Factor 3)
RN  - 0 (Anesthetics, Local)
RN  - 0 (Atf3 protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GAP-43 Protein)
RN  - 4368-28-9 (Tetrodotoxin)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Activating Transcription Factor 3/metabolism
MH  - Anesthetics, Local/pharmacology
MH  - Animals
MH  - Axons/physiology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Count
MH  - Cell Proliferation
MH  - Electric Stimulation
MH  - Female
MH  - Femoral Nerve/cytology/physiology
MH  - GAP-43 Protein/metabolism
MH  - Gene Expression/*physiology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Nerve Regeneration/*physiology
MH  - Neurons, Afferent/*physiology
MH  - Perfusion
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tetrodotoxin/pharmacology
MH  - Tissue Fixation
EDAT- 2007/04/13 09:00
MHDA- 2007/07/11 09:00
CRDT- 2007/04/13 09:00
PHST- 2006/09/15 00:00 [received]
PHST- 2007/01/16 00:00 [revised]
PHST- 2007/01/19 00:00 [accepted]
PHST- 2007/04/13 09:00 [pubmed]
PHST- 2007/07/11 09:00 [medline]
PHST- 2007/04/13 09:00 [entrez]
AID - S0014-4886(07)00042-8 [pii]
AID - 10.1016/j.expneurol.2007.01.040 [doi]
PST - ppublish
SO  - Exp Neurol. 2007 Jun;205(2):347-59. doi: 10.1016/j.expneurol.2007.01.040. Epub 
      2007 Feb 21.