PMID- 17428632
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20070528
IS  - 0734-9750 (Print)
IS  - 0734-9750 (Linking)
VI  - 25
IP  - 4
DP  - 2007 Jul-Aug
TI  - Intelligent bioprocessing for haemotopoietic cell cultures using monitoring and 
      design of experiments.
PG  - 353-68
AB  - The need for successful ex-vivo expansion and directed differentiation of 
      haematopoietic stem cells (HSCs) for therapeutic applications has increased over 
      the past decade. Haematopoietic cell cultures are complex and full 
      characterisation of the process environment has yet to be achieved. The 
      complexity and transient nature of HSC cultures make the identification, 
      maintenance and control of optimal operating conditions challenging. Application 
      of real-time, on-line monitoring techniques and process control strategies 
      enhances the ability to operate bioprocesses of desired reproducibility and high 
      product quality. In this review, we discussed the methods by which in vitro 
      culture information necessary for bioprocess control may be obtained, including 
      process considerations, monitoring and analytical tools, and design of 
      experiments (DOE). The successful application of these tools may result in time- 
      and cost-effective cultures for directed differentiation and expansion of 
      haematopoietic components intended for clinical use.
FAU - Lim, Mayasari
AU  - Lim M
AD  - Biological Systems Engineering Laboratory, Department of Chemical Engineering, 
      Imperial College London, SW7 2AZ, United Kingdom.
FAU - Ye, Hua
AU  - Ye H
FAU - Panoskaltsis, Nicki
AU  - Panoskaltsis N
FAU - Drakakis, Emmanuel M
AU  - Drakakis EM
FAU - Yue, Xicai
AU  - Yue X
FAU - Cass, Anthony E G
AU  - Cass AE
FAU - Radomska, Anna
AU  - Radomska A
FAU - Mantalaris, Athanasios
AU  - Mantalaris A
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20070215
PL  - England
TA  - Biotechnol Adv
JT  - Biotechnology advances
JID - 8403708
SB  - IM
MH  - Animals
MH  - Biotechnology/*instrumentation/*methods
MH  - Cell Culture Techniques/*methods
MH  - Cell Proliferation
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
RF  - 81
EDAT- 2007/04/13 09:00
MHDA- 2007/08/02 09:00
CRDT- 2007/04/13 09:00
PHST- 2006/08/17 00:00 [received]
PHST- 2007/01/26 00:00 [revised]
PHST- 2007/02/05 00:00 [accepted]
PHST- 2007/04/13 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/04/13 09:00 [entrez]
AID - S0734-9750(07)00028-6 [pii]
AID - 10.1016/j.biotechadv.2007.02.002 [doi]
PST - ppublish
SO  - Biotechnol Adv. 2007 Jul-Aug;25(4):353-68. doi: 10.1016/j.biotechadv.2007.02.002. 
      Epub 2007 Feb 15.