PMID- 17431094
OWN - NLM
STAT- MEDLINE
DCOM- 20071105
LR  - 20181201
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 21
IP  - 10
DP  - 2007 Aug
TI  - HIV-1 Nef impairs the dynamic of DC/NK crosstalk: different outcome of CD56(dim) 
      and CD56(bright) NK cell subsets.
PG  - 2323-34
AB  - Dendritic cells (DCs) and natural killer (NK) cells are essential components of 
      the innate immunity and play a critical role in the first phase of host defense 
      against infection. Interactions between DCs and NK cells have been demonstrated 
      in a variety of settings, with evidence emerging of complex bidirectional 
      crosstalk between the two cell types. The accessory HIV-1 Nef protein is a 
      crucial determinant for viral replication and pathogenesis. We previously 
      demonstrated that Nef, hijacking DC functional activity, subverts the DC arm of 
      immune response to escape the adaptive immune attack. Here, we monitor the effect 
      of Nef on the outcome of the innate immune response, focusing on the impact of 
      Nef on DC/NK crosstalk. We demonstrate that Nef up-regulates the ability of DCs 
      to stimulate the immunoregulatory NK cells (CD56(bright)) as assessed by the 
      activated phenotype, up-regulation of their proliferative response and INF-gamma 
      release. On the other hand, Nef-pulsed DCs inhibit cytotoxic NK cells 
      (CD56(dim)), as assessed by the reduced HLA-DR surface expression, reduced 
      proliferation and cytotoxic activity. Moreover, in the presence of Nef-pulsed 
      DCs, we found a significant up-regulation of TNF-alpha secretion and a 
      significant reduction of IL-10, GM-CSF, MIP-1alpha and RANTES secretion. Our 
      findings suggest that the Nef-induced dysregulation in the DC/NK cell crosstalk 
      may represent a potential mechanism through which HIV escapes innate immune 
      surveillance.
FAU - Quaranta, Maria Giovanna
AU  - Quaranta MG
AD  - Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Viale 
      Regina Elena, 299, 00161 Rome, Italy.
FAU - Napolitano, Alfonso
AU  - Napolitano A
FAU - Sanchez, Massimo
AU  - Sanchez M
FAU - Giordani, Luciana
AU  - Giordani L
FAU - Mattioli, Benedetta
AU  - Mattioli B
FAU - Viora, Marina
AU  - Viora M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070412
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (CD56 Antigen)
RN  - 0 (Gene Products, nef)
RN  - 0 (Recombinant Proteins)
RN  - 0 (nef Gene Products, Human Immunodeficiency Virus)
RN  - 130068-27-8 (Interleukin-10)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/immunology
MH  - CD56 Antigen/classification/drug effects/*physiology
MH  - Cell Division
MH  - Dendritic Cells/drug effects/*immunology/virology
MH  - Flow Cytometry
MH  - Gene Products, nef/*pharmacology/physiology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Interleukin-10/metabolism
MH  - Killer Cells, Natural/drug effects/*immunology/virology
MH  - Recombinant Proteins/pharmacology
MH  - Virus Replication/physiology
MH  - nef Gene Products, Human Immunodeficiency Virus
EDAT- 2007/04/14 09:00
MHDA- 2007/11/06 09:00
CRDT- 2007/04/14 09:00
PHST- 2007/04/14 09:00 [pubmed]
PHST- 2007/11/06 09:00 [medline]
PHST- 2007/04/14 09:00 [entrez]
AID - fj.06-7883com [pii]
AID - 10.1096/fj.06-7883com [doi]
PST - ppublish
SO  - FASEB J. 2007 Aug;21(10):2323-34. doi: 10.1096/fj.06-7883com. Epub 2007 Apr 12.