PMID- 17431675
OWN - NLM
STAT- MEDLINE
DCOM- 20070828
LR  - 20191210
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 450
IP  - 5
DP  - 2007 May
TI  - Specific activation of the different fibrogenic cells in rat cultured liver 
      slices mimicking in vivo situations.
PG  - 503-12
AB  - Due to the loss of cell-cell and cell-matrix interactions, cell culture models 
      poorly mimic the in vivo situation. Therefore, we tested the applicability of 
      precision-cut liver slices (PCLS) to study the early activation of the two main 
      liver fibrogenic cell subpopulations: hepatic stellate cells (HSC) and portal 
      fibroblasts (PF). PCLS were treated with thioacetamide or acetaminophen to induce 
      HSC activation. In PCLS culture, both were able to trigger centrolobular lesion 
      and HSC activation as observed in vivo. However, thioacetamide also presented a 
      toxic effect on portal tract cells. In this PCLS model of centrolobular lesion, 
      the antioxidant N-acetylcysteine was able to prevent acetaminophen-induced 
      injury. To induce a specific activation of PF, PCLS were treated with epidermal 
      growth factor or beta-oestradiol. As in vivo, epidermal growth factor and 
      beta-oestradiol induced bile duct epithelial cell proliferation accompanied by PF 
      activation; however, beta-oestradiol also triggers sinusoidal cell proliferation. 
      We demonstrated that treatments usually used in vivo to induce liver fibrosis 
      allow, in cultured PCLS, the specific activation of the two main liver fibrogenic 
      cell subpopulations, making this model very useful to study the mechanisms 
      involved in early fibrogenic cell activation.
FAU - Guyot, Christelle
AU  - Guyot C
AD  - INSERM, E362, Universite Victor Segalen Bordeaux 2, Bordeaux, 33076, France.
FAU - Combe, Chantal
AU  - Combe C
FAU - Clouzeau-Girard, Haude
AU  - Clouzeau-Girard H
FAU - Moronvalle-Halley, Valerie
AU  - Moronvalle-Halley V
FAU - Desmouliere, Alexis
AU  - Desmouliere A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20070313
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Antioxidants)
RN  - 075T165X8M (Thioacetamide)
RN  - 362O9ITL9D (Acetaminophen)
RN  - 4TI98Z838E (Estradiol)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetaminophen/toxicity
MH  - Acetylcysteine/pharmacology
MH  - Animal Use Alternatives
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Bile Ducts, Intrahepatic/drug effects/pathology
MH  - Cell Survival/drug effects
MH  - *Disease Models, Animal
MH  - Drug Antagonism
MH  - Epidermal Growth Factor/pharmacology
MH  - Estradiol/pharmacology
MH  - Fibroblasts/drug effects/metabolism/*pathology
MH  - Hepatocytes/drug effects/pathology
MH  - Kupffer Cells/drug effects/metabolism/*pathology
MH  - Liver/drug effects/metabolism/*pathology
MH  - Liver Cirrhosis/chemically induced/pathology
MH  - Male
MH  - Necrosis
MH  - Organ Culture Techniques
MH  - Portal System/drug effects/metabolism/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Thioacetamide/toxicity
EDAT- 2007/04/14 09:00
MHDA- 2007/08/29 09:00
CRDT- 2007/04/14 09:00
PHST- 2006/12/12 00:00 [received]
PHST- 2007/02/12 00:00 [accepted]
PHST- 2007/02/06 00:00 [revised]
PHST- 2007/04/14 09:00 [pubmed]
PHST- 2007/08/29 09:00 [medline]
PHST- 2007/04/14 09:00 [entrez]
AID - 10.1007/s00428-007-0390-y [doi]
PST - ppublish
SO  - Virchows Arch. 2007 May;450(5):503-12. doi: 10.1007/s00428-007-0390-y. Epub 2007 
      Mar 13.