PMID- 17432580
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 1
DP  - 2007 Jan
TI  - [Analysis of monosaccharides and uronic acids in polysaccharides by pre-column 
      derivatization with p-aminobenzoic acid and high performance liquid 
      chromatography].
PG  - 75-9
AB  - An ion-pair reversed-phase high performance liquid chromatographic (RP-HPLC) 
      method for the simultaneous determination of carbohydrate and uronic acids was 
      developed. p-Aminobenzoic acid (p-AMBA) was used for pre-column derivatization of 
      the analytes, enabling fluorescence (lambda(ex) = 313 nm, lambda(em) = 358 nm) or 
      ultraviolet (UV at 303 nm) detection. Reaction conditions such as reaction 
      temperature and reaction time were optimized. Atlantis dC18 column with 
      hydrophilic end capping was selected for the separation of derivatives. Effects 
      of mobile phase compositions such as ion pairs and their concentrations and pH on 
      the retention behaviors and separation results of 9 monosaccharides and 2 uronic 
      acids were investigated. Derivatives of fructose, galactose, glucose, mannose, 
      xylose, arabinose, ribose, galacturonic acid, fucose, glucuronic acid and 
      rhamnose were separated within 42 min, applying tetrabutyl ammonium hydrogen 
      bisulfate (TBAHSO4) as the ion pair reagent. The detection limits were between 
      3.38 x 10(-8) mol/L and 176 x 10(-8) mol/L for fluorescence detection and between 
      2.55 x 10(-7) mol/L and 13.4 x 10(-7) mol/L for UV detection. Good linearities 
      were obtained with correlation coefficients (r2) above 0.99. The relative 
      standard deviations (RSDs) of the peak area of the derivatives in 12 - 51 h after 
      derivatization were from 2.5% to 3.9%. This method has been applied for the 
      determination of mono-/disaccharides and uronic acids in spirulina polysaccharide 
      after dissolved in trifluoroacetic acid solution (2 mol/L). The results showed 
      this method is suitable for the analysis of monosaccharide compositions in 
      polysaccharides.
FAU - Hao, Guitang
AU  - Hao G
AD  - School of Chemistry and Material Engineering of Southern Yangtze University, Wuxi 
      214036, China.
FAU - Chen, Shangwei
AU  - Chen S
FAU - Zhu, Song
AU  - Zhu S
FAU - Yin, Hongping
AU  - Yin H
FAU - Dai, Jun
AU  - Dai J
FAU - Cao, Yuhua
AU  - Cao Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Monosaccharides)
RN  - 0 (Polysaccharides)
RN  - 0 (Uronic Acids)
RN  - TL2TJE8QTX (4-Aminobenzoic Acid)
SB  - IM
MH  - 4-Aminobenzoic Acid/*chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Hydrogen-Ion Concentration
MH  - Molecular Structure
MH  - Monosaccharides/analysis/*chemistry
MH  - Polysaccharides/*chemistry
MH  - Reproducibility of Results
MH  - Uronic Acids/analysis/*chemistry
EDAT- 2007/04/17 09:00
MHDA- 2010/06/11 06:00
CRDT- 2007/04/17 09:00
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 Jan;25(1):75-9.