PMID- 17432585
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 1
DP  - 2007 Jan
TI  - [Study on capillary electrophoresis fingerprints of Flos Lonicerae Japonicae].
PG  - 96-100
AB  - The capillary electrophoresis fingerprints (CEFP) of Flos Lonicerae Japonicae was 
      established to control its quality. In the capillary zone electrophoresis (CZE) 
      pattern 50 mmol/L sodium borate contained 20 mmol/L beta-cyclodextrin (beta-CD) 
      adjusted to pH 8.0 with phosphoric acid was applied as the background 
      electrolyte. The running voltage was 12 kV and the detection wavelength was 254 
      nm. The Flos Lonicerae Japonicae was extracted by water and the sample solution 
      was injected into the capillary by hydraulic pressure in 15 s. The 18 common 
      peaks were marked according to the emerging rate of 100% and by comparing each 
      fingerprint with the other one among the 13 samples cultivated in different 
      places, in which chlorogenic acid was selected as the reference peak. The CEFP 
      had good precision and reproducibility with the relative standard deviations 
      (RSDs) of the relative migration times less than 3% and the RSDs of the relative 
      peak areas within 6.1%. The novel concepts of the apparent quantitative 
      similarity (R), the quantitative similarity calculated by vector projection (C) 
      and the quantitative similarity (P) were introduced firstly. The good crude drugs 
      should posses two merits as follows: the qualitative similarity (S) which 
      displays the distribution of the chemical constituents in sample should be more 
      than 0.90; the quantitative similarities (R, C, P, Q) that disclose the overall 
      contents of the chemical constituents in sample should be within 80% - 120%. This 
      method was applied in the quality control practice, and the results showed that 
      the method could be used for the overall quality control of Flos Lonicerae 
      Japonicae and is especially suitable for qualitative and quantitative evaluation 
      of chromatographic fingerprints both in chemical constituent distribution and in 
      contents.
FAU - Sun, Guoxiang
AU  - Sun G
AD  - College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. 
      gxswmwys@163.com
FAU - Yang, Hongtao
AU  - Yang H
FAU - Deng, Xiangyu
AU  - Deng X
FAU - Sun, Yuqing
AU  - Sun Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - Lonicera/*chemistry
MH  - Reproducibility of Results
EDAT- 2007/04/17 09:00
MHDA- 2010/06/11 06:00
CRDT- 2007/04/17 09:00
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 Jan;25(1):96-100.