PMID- 17432610
OWN - NLM
STAT- MEDLINE
DCOM- 20070626
LR  - 20131121
IS  - 0041-3771 (Print)
IS  - 0041-3771 (Linking)
VI  - 49
IP  - 1
DP  - 2007
TI  - [Prooxidant and cytotoxic action of N-acetylcysteine and glutathione combined 
      with vitamin Bl2b].
PG  - 70-8
AB  - We studied the prooxidant and cytotoxic action of thiols N-acetylcystein (NAC) 
      and glutathione (GSH) combined with vitamin Bl2b. The synergism of action of the 
      thiols and Bl2b resulted in human carcinoma cell damage was found. It was shown 
      that GSH and NAC in physiological doses combined with Bl2b caused the initiation 
      of apoptosis. It was established that prooxidant action of the thiols combined 
      with vitamin Bl2b, i. e. generation and accumulation of hydrogen peroxide in 
      culture medium, led to intracellular oxidative stress and injury of cell redox 
      system. These effects were completely abolished by nonthiol antioxidants catalase 
      and pyruvate. The chelators of iron phenanthroline and deferoxamine did not 
      suppress the H2O2 accumulation in culture medium but significantly inhibited the 
      cell death induced by the thiols combined with Bl2b. Therefore, the thiols GSH 
      and NAC widely used as antioxidants, in combination with vitamin Bl2b show 
      prooxidant characteristics and induce, with the participation of intracellular 
      iron, apoptotic HEp-2 cell death.
FAU - Solov'eva, M E
AU  - Solov'eva ME
FAU - Solov'ev, V V
AU  - Solov'ev VV
FAU - Faskhutdinova, A A
AU  - Faskhutdinova AA
FAU - Kudriavtsev, A A
AU  - Kudriavtsev AA
FAU - Akatov, V S
AU  - Akatov VS
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Tsitologiia
JT  - Tsitologiia
JID - 0417363
RN  - 0 (Antioxidants)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Iron Compounds)
RN  - 0 (Phenanthrolines)
RN  - 0 (Siderophores)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - EC 1.11.1.6 (Catalase)
RN  - GAN16C9B8O (Glutathione)
RN  - J06Y7MXW4D (Deferoxamine)
RN  - P6YC3EG204 (Vitamin B 12)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*pharmacology
MH  - Antioxidants/pharmacology
MH  - *Apoptosis/drug effects
MH  - Catalase/pharmacology
MH  - Cell Line, Tumor
MH  - Cell Physiological Phenomena/*drug effects
MH  - Deferoxamine/pharmacology
MH  - Drug Synergism
MH  - Free Radical Scavengers/*pharmacology
MH  - Glutathione/*pharmacology
MH  - Humans
MH  - Iron Compounds/pharmacology
MH  - Oxidation-Reduction
MH  - Oxidative Stress/drug effects
MH  - Phenanthrolines/pharmacology
MH  - Pyruvic Acid/pharmacology
MH  - Siderophores/pharmacology
MH  - Vitamin B 12/*pharmacology
EDAT- 2007/04/17 09:00
MHDA- 2007/06/27 09:00
CRDT- 2007/04/17 09:00
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2007/06/27 09:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
PST - ppublish
SO  - Tsitologiia. 2007;49(1):70-8.