PMID- 17433345
OWN - NLM
STAT- MEDLINE
DCOM- 20071003
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1159
IP  - 1-2
DP  - 2007 Aug 3
TI  - Pressurized liquid extraction combined with capillary electrophoresis-mass 
      spectrometry as an improved methodology for the determination of sulfonamide 
      residues in meat.
PG  - 233-41
AB  - A new analytical method, based on capillary electrophoresis and tandem mass 
      spectrometry (CE-MS2), is proposed and validated for the identification and 
      simultaneous quantification of 12 sulfonamides (SAs) in pork meat. The studied 
      SAs include sulfathiazole, sulfadiazine, sulfamethoxypyridazine, sulfaguanidine, 
      sulfanilamide, sulfadimethoxyne, sulfapyridine, sulfachloropyridazine, 
      sulfisoxazole, sulfasalazine, sulfabenzamide and sulfadimidine. Different 
      parameters (i.e. separation buffer, sheath liquid, electrospray conditions) were 
      optimized to obtain an adequate CE separation and high MS sensitivity. MS2 
      experiments using an ion trap as analyzer, operating in the selected reaction 
      monitoring (SRM) mode, were carried out to achieve the required number of 
      identification points according to the 2002/657/EC European Decision. For the 
      quantification in pork tissue samples, a pressurized liquid extraction (PLE) 
      procedure, using hot water as extractant followed by an Oasis HLB cleanup, was 
      developed. Linearity (r between 0.996 and 0.997), precision (RSD<14 %) and 
      recoveries (from 76 to 98%) were satisfactory. The limits of detection and 
      quantification (below 12.5 and 46.5 microg kg(-1), respectively) were in all 
      cases lower than the maximum residue limits (MRLs), indicating the potential of 
      CE-MS2 for the analysis of SAs, in the food quality and safety control areas.
FAU - Font, Guillermina
AU  - Font G
AD  - Laboratori de Bromatologia i Toxicologia, Facultat de Farmacia, Universitat de 
      Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain.
FAU - Juan-Garcia, Ana
AU  - Juan-Garcia A
FAU - Pico, Yolanda
AU  - Pico Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20070321
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Complex Mixtures)
RN  - 0 (Sulfonamides)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Animals
MH  - Calibration
MH  - Chromatography, Liquid/methods
MH  - Complex Mixtures/analysis
MH  - Electrophoresis, Capillary/instrumentation/*methods
MH  - Food Analysis/*methods
MH  - Food Contamination/*prevention & control
MH  - Meat/*analysis
MH  - Pressure
MH  - Quality Control
MH  - Sensitivity and Specificity
MH  - Sulfonamides/*analysis
MH  - Swine
MH  - Tandem Mass Spectrometry/instrumentation/*methods
MH  - Water
EDAT- 2007/04/17 09:00
MHDA- 2007/10/04 09:00
CRDT- 2007/04/17 09:00
PHST- 2007/01/26 00:00 [received]
PHST- 2007/03/07 00:00 [revised]
PHST- 2007/03/19 00:00 [accepted]
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2007/10/04 09:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
AID - S0021-9673(07)00543-2 [pii]
AID - 10.1016/j.chroma.2007.03.062 [doi]
PST - ppublish
SO  - J Chromatogr A. 2007 Aug 3;1159(1-2):233-41. doi: 10.1016/j.chroma.2007.03.062. 
      Epub 2007 Mar 21.